Construct: ORF TRCN0000487941
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019508.1_s317c1
- DNA Barcode:
- TAACGAGACTGACCACGCATAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TESK2 (10420)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487941
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10420 | TESK2 | testis associated actin rem... | NM_007170.3 | 94.5% | 94.5% | 655_657delTCCinsGAG;793_879del;1711_1713delGGG |
| 2 | human | 10420 | TESK2 | testis associated actin rem... | NM_001320800.2 | 80% | 80% | (many diffs) |
| 3 | mouse | 230661 | Tesk2 | testis-specific kinase 2 | NM_146151.4 | 82.1% | 82.6% | (many diffs) |
| 4 | mouse | 230661 | Tesk2 | testis-specific kinase 2 | XM_006503008.3 | 64.2% | 64% | (many diffs) |
| 5 | mouse | 230661 | Tesk2 | testis-specific kinase 2 | XR_001784124.1 | 41.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 1716
- ORF length:
- 1623
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaggct 61 ccgcggccgc ccccttcacc tctagacaga ctatggatcg gagcaaacgg aattcaattg 121 caggatttcc tccacgtgtg gagcgtcttg aagagtttga aggaggtggt ggaggagaag 181 gaaatgtgag ccaggtggga agagtttggc catcttcgta tcgagctctt ataagtgcct 241 tttccagact gacgcgtttg gatgatttca cctgtgaaaa aatagggtct ggcttctttt 301 ctgaagtgtt caaggtacga caccgagctt ctggtcaggt gatggctctt aagatgaaca 361 cattgagcag taaccgggca aacatgctga aagaagtaca gctcatgaat agactctccc 421 atcccaacat ccttaggttc atgggtgtat gtgttcatca aggacaattg catgcactta 481 cagagtatat caactccggg aacctggaac agttgctaga cagtaacctg catttgcctt 541 ggactgtgag ggtaaaactg gcctatgaca tagcagtggg cctcagctac cttcacttca 601 aaggcatttt tcatcgggac ctcacatcta agaactgcct gataaagagg gatgagaatg 661 gttactctgc agtggtagct gactttggcc tggctgagaa gatccccgat gtcagcatgg 721 ggagtgagaa gctggccgtg gtgggtgagc cattctggat ggcacctgag gttctccgag 781 atgagcccta taatgaaaag gcagatgtgt tctcttatgg tatcatcctc tgcgagatca 841 tcgcccgcat ccaggccgat ccggactatc ttccccgcac agagatggat cccaaactgc 901 gcccatcttt tgtggagatt gggaagaccc tggaggaaat tctgagccgc ctacaggaag 961 aagagcagga gagggatagg aagctgcagc ccacagccag gggactcttg gagaaagcac 1021 ctggggtgaa gcgactaagc tcactggatg acaagatccc ccacaagtca ccatgcccaa 1081 gacgtaccat ctggctgtct cgaagccagt cagatatctt ttcccgtaag cccccacgta 1141 cagtgagtgt cttggaccca tactaccggc cacgagatgg tgctgcccgc acccccaaag 1201 tcaacccttt tagtgctcgc caggacctca tggggggcaa gatcaagttt tttgacctgc 1261 ccagcaagtc tgtcatctct ctggtatttg acctggatgc accagggccc ggaactatgc 1321 ccctggctga ctggcaggag cccctggccc cacctattcg ccggtggcgt tccttgcctg 1381 gttcgcctga gttcttgcat caagaggctt gtccatttgt gggccgggaa gaatcgctat 1441 ctgatgggcc cccaccacgc cTAAGTAGTC TCAAGTACAG AGTTAAAGAG ATCCCACCAT 1501 TCCGGGCATC TGCCCTACCA GCTGCTCAAG CCCATGAGGC TATGGACTGC TCCATTCTCC 1561 AGGAAGAAAA TGGTTTTGGG TCCAGGCCCC AGGGGACCAG TCCATGCCCT GCGGGTGCTT 1621 CTGAGGAGAT GGAGGTAGAA GAAAGGCCAG CAGGCTCAAC TCCAGCCACC TTCTCCACCT 1681 CAGGCATAGG CCTGCAAACC CAGGGAAAGC AGGATAAGGG TGGGCGCGCC GACCCAGCTT 1741 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1801 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1861 CTTGTGGAAA GGACGATAAC GAGACTGACC ACGCATAACT ACGCGTTAAG TCgacaatca 1921 acctctggat tacaaaattt gtgaaagatt