Construct: ORF TRCN0000487956
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021395.1_s317c1
- DNA Barcode:
- CATCCGTGGTTTGCCCTTCTGGGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RDH12 (145226)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487956
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 145226 | RDH12 | retinol dehydrogenase 12 | NM_152443.3 | 99% | 99.6% | 482G>A;948_949insTAGAAGCT |
| 2 | mouse | 77974 | Rdh12 | retinol dehydrogenase 12 | NM_030017.4 | 82.3% | 86% | (many diffs) |
| 3 | mouse | 77974 | Rdh12 | retinol dehydrogenase 12 | NM_001313971.1 | 79% | 82.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1014
- ORF length:
- 948
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaat 61 tcaccatgct ggtcaccttg ggactgctca cctccttctt ctcgttcctg tatatggtag 121 ctccatccat caggaagttc tttgctggtg gagtgtgtag aacaaatgtg cagcttcctg 181 gcaaggtagt ggtgatcact ggcgccaaca cgggcattgg caaggagacg gccagagagc 241 tcgctagccg aggagcccga gtctatattg cctgcagaga tgtactgaag ggggagtctg 301 ctgccagtga aatccgagtg gatacaaaga actcccaggt gctggtgcgg aaattggacc 361 tatccgacac caaatctatc cgagcctttg ctgagggctt tctggcagag gaaaagcagc 421 tccatattct gatcaacaat gcgggagtaa tgatgtgtcc atattccaag acagctgatg 481 gctttgaaac ccacctggga gtcaaccacc tgggccactt cctcctcacc tacctgctcc 541 tggagcagct aaaggtgtct gcccctgcac gggtggttaa tgtgtcctcg gtggctcacc 601 acattggcaa gattcccttc cacgacctcc agagcgagaa gcgctacagc aggggttttg 661 cctattgcca cagcaagctg gccaatgtgc tttttactcg tgagctggcc aagaggctcc 721 aaggcaccgg ggtcaccacc TACGCAGTGC ACCCAGGCGT CGTCCGCTCT GAGCTGGTCC 781 GGCACTCCTC CCTGCTCTGC CTGCTCTGGC GGCTCTTCTC CCCCTTTGTC AAGACGGCAC 841 GGGAGGGGGC GCAGACCAGC CTGCACTGCG CCCTGGCTGA GGGCCTGGAG CCCCTGAGTG 901 GCAAGTACTT CAGTGACTGC AAGAGGACCT GGGTGTCTCC AAGGGCCCGA AATAACAAAA 961 CAGCTGAGCG CCTATGGAAT GTCAGCTGTG AGCTTCTAGG AATCCGGTGG GAGTAGAAGC 1021 TTGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1081 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1141 GGCTTTATAT ATCTTGTGGA AAGGACGACA TCCGTGGTTT GCCCTTCTGG GCACGCGTTA 1201 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt