Construct: ORF TRCN0000487977
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021361.1_s317c1
- DNA Barcode:
- ATCGCCAACAATTATACGAACAAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MMP28 (79148)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487977
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79148 | MMP28 | matrix metallopeptidase 28 | NM_001032278.3 | 100% | 100% | |
| 2 | human | 79148 | MMP28 | matrix metallopeptidase 28 | XM_017025062.1 | 33.9% | 33.5% | (many diffs) |
| 3 | human | 79148 | MMP28 | matrix metallopeptidase 28 | XM_017025063.1 | 33.9% | 33.5% | (many diffs) |
| 4 | human | 79148 | MMP28 | matrix metallopeptidase 28 | XM_017025064.1 | 33.9% | 33.5% | (many diffs) |
| 5 | human | 79148 | MMP28 | matrix metallopeptidase 28 | XM_011525231.1 | 32.9% | 32.5% | (many diffs) |
| 6 | human | 79148 | MMP28 | matrix metallopeptidase 28 | NM_032950.4 | 32.6% | 32.3% | (many diffs) |
| 7 | human | 79148 | MMP28 | matrix metallopeptidase 28 | XM_011525230.1 | 30.7% | 30.3% | (many diffs) |
| 8 | human | 79148 | MMP28 | matrix metallopeptidase 28 | XM_011525225.1 | 28.9% | 28.7% | (many diffs) |
| 9 | human | 79148 | MMP28 | matrix metallopeptidase 28 | XM_011525232.2 | 25.9% | 25.7% | (many diffs) |
| 10 | human | 79148 | MMP28 | matrix metallopeptidase 28 | NM_024302.5 | 24.6% | 24.4% | (many diffs) |
| 11 | human | 79148 | MMP28 | matrix metallopeptidase 28 | NR_111988.2 | 16.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 462
- ORF length:
- 390
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggtcgcg cgcgtcggcc tccTGCTGCG CGCCCTGCAG CTGCTACTGT 121 GGGGCCACCT GGACGCCCAG CCCGCGGAGC GCGGAGGCCA GGAGCTGCGC AAGGAGGCGG 181 AGGCATTCCT AGAGAAGTAC GGATACCTCA ATGAACAGGT CCCCAAAGCT CCCACCTCCA 241 CTCGATTCAG CGATGCCATC AGAGCGTTTC AGTGGGTGTC CCAGCTACCT GTCAGCGGCG 301 TGTTGGACCG CGCCACCCTG CGCCAGATGA CTCGTCCCCG CTGCGGGGTT ACAGATACCA 361 ACAGTTATGC GGCCTGGGCT GAGAGGATCA GTGACTTGTT TGCTAGACAC CGGACCAAAA 421 TGAGGCGTAA GAAACGCTTT GCAAAGCAAG GTGAGCACTG TTAGAACCCA GCTTTCTTGT 481 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 541 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 601 GAAAGGACGA ATCGCCAACA ATTATACGAA CAACACGCGT TAAGTCgaca atcaacctct 661 ggattacaaa atttgtgaaa gatt