Construct: ORF TRCN0000487990

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019612.1_s317c1
DNA Barcode:
GCCTGACAAAGAGTAAAACGACGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PTGER2 (5732)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487990
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5732 PTGER2 prostaglandin E receptor 2 NM_000956.4 99.9% 99.7% 1074_1075insG
2 mouse 19217 Ptger2 prostaglandin E receptor 2 ... NM_008964.4 83% 85.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
75
ORF end:
1152
ORF length:
1077
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcgc caccatgggc aatgcctcca atgactccca gtctgaggac tgcgagacgc
121gacagtggct tcccccaggc gaaagcccag ccatcagctc cgtcatgttc tcggccgggg
181tgctggggaa cctcatagca ctggcgctgc tggcgcgccg ctggcggggg gacgtggggt
241gcagcgccgg ccgcaggagc tccctctcct tgttccacgt gctggtgacc gagctggtgt
301tcaccgacct gctcgggacc tgcctcatca gcccagtggt actggcttcg tacgcgcgga
361accagaccct ggtggcactg gcgcccgaga gccgcgcgtg cacctacttc gctttcgcca
421tgaccttctt cagcctggcc acgatgctca tgctcttcgc catggccctg gagcgctacc
481tctcgatcgg gcacccctac ttctaccagc gccgcgtctc gcgctccggg ggcctggccg
541tgctgcctgt catctatgca gtctccctgc tcttctgctc gctgccgctg ctggactatg
601ggcagtacgt ccagtactgc cccgggacct ggtgcttcat ccggcacggg cggaccgctt
661acctgcagct gtacgccacc ctgctgctgc ttctcattgt ctcggtgctc gcctgcaact
721tcagtgtcat tctcaacctc atccgcatgc accgccgaag ccggagaagc cgctgcggac
781cttccctggg cagtggccgg ggcggccccg gggcccgcag gagagggGAA AGGGTGTCCA
841TGGCGGAGGA GACGGACCAC CTCATTCTCC TGGCTATCAT GACCATCACC TTCGCCGTCT
901GCTCCTTGCC TTTCACGATT TTTGCATATA TGAATGAAAC CTCTTCCCGA AAGGAAAAAT
961GGGACCTCCA AGCTCTTAGG TTTTTATCAA TTAATTCAAT AATTGACCCT TGGGTCTTTG
1021CCATCCTTAG GCCTCCTGTT CTGAGACTAA TGCGTTCAGT CCTCTGTTGT CGGATTTCAT
1081TAAGAACACA AGATGCAACA CAAACTTCCT GTTCTACACA GTCAGATGCC AGTAAACAGG
1141CTGACCTTGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1201CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1261TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGCCTGA CAAAGAGTAA AACGACGAAC
1321GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)