Construct: ORF TRCN0000487990
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019612.1_s317c1
- DNA Barcode:
- GCCTGACAAAGAGTAAAACGACGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTGER2 (5732)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487990
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 5732 | PTGER2 | prostaglandin E receptor 2 | NM_000956.4 | 99.9% | 99.7% | 1074_1075insG |
2 | mouse | 19217 | Ptger2 | prostaglandin E receptor 2 ... | NM_008964.4 | 83% | 85.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1152
- ORF length:
- 1077
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgggc aatgcctcca atgactccca gtctgaggac tgcgagacgc 121 gacagtggct tcccccaggc gaaagcccag ccatcagctc cgtcatgttc tcggccgggg 181 tgctggggaa cctcatagca ctggcgctgc tggcgcgccg ctggcggggg gacgtggggt 241 gcagcgccgg ccgcaggagc tccctctcct tgttccacgt gctggtgacc gagctggtgt 301 tcaccgacct gctcgggacc tgcctcatca gcccagtggt actggcttcg tacgcgcgga 361 accagaccct ggtggcactg gcgcccgaga gccgcgcgtg cacctacttc gctttcgcca 421 tgaccttctt cagcctggcc acgatgctca tgctcttcgc catggccctg gagcgctacc 481 tctcgatcgg gcacccctac ttctaccagc gccgcgtctc gcgctccggg ggcctggccg 541 tgctgcctgt catctatgca gtctccctgc tcttctgctc gctgccgctg ctggactatg 601 ggcagtacgt ccagtactgc cccgggacct ggtgcttcat ccggcacggg cggaccgctt 661 acctgcagct gtacgccacc ctgctgctgc ttctcattgt ctcggtgctc gcctgcaact 721 tcagtgtcat tctcaacctc atccgcatgc accgccgaag ccggagaagc cgctgcggac 781 cttccctggg cagtggccgg ggcggccccg gggcccgcag gagagggGAA AGGGTGTCCA 841 TGGCGGAGGA GACGGACCAC CTCATTCTCC TGGCTATCAT GACCATCACC TTCGCCGTCT 901 GCTCCTTGCC TTTCACGATT TTTGCATATA TGAATGAAAC CTCTTCCCGA AAGGAAAAAT 961 GGGACCTCCA AGCTCTTAGG TTTTTATCAA TTAATTCAAT AATTGACCCT TGGGTCTTTG 1021 CCATCCTTAG GCCTCCTGTT CTGAGACTAA TGCGTTCAGT CCTCTGTTGT CGGATTTCAT 1081 TAAGAACACA AGATGCAACA CAAACTTCCT GTTCTACACA GTCAGATGCC AGTAAACAGG 1141 CTGACCTTGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1201 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1261 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGCCTGA CAAAGAGTAA AACGACGAAC 1321 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt