Construct: ORF TRCN0000488005
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021264.1_s317c1
- DNA Barcode:
- CACCCGCTTATAAACCTACGGCGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LOC115131 (115131)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488005
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 8587 | OR7E2P | olfactory receptor family 7... | NR_045004.1 | 45.1% | (many diffs) | |
2 | human | 79315 | OR7E91P | olfactory receptor family 7... | NR_002185.3 | 39.9% | 1_402del;904_1255del | |
3 | human | 219445 | OR7E5P | olfactory receptor family 7... | NR_027688.2 | 24.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 573
- ORF length:
- 501
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtgcttc ttcctctcca acctgtgctg ggctgacatc ggtttcacct 121 cggccacggt tcccaagatg attgtggaca tgcggtcgca tagcggagtc atctcttatg 181 cggactgcct gacacggatg tctttcttgg tcctttttgc atgtgtagaa gacatgctcc 241 tgactgtgat ggcctatgac tgttttGTAG CCATCTGTCG CCCTCTGCAC TACCCAGTCA 301 TCGTGAATCC TCACCTCTGT GTCTTCTTAG TTTCGGTGTC CTTTTCCTTA GCCTGTTGGA 361 TTCCCAGCTG CGCAGTTGGA TTGTGTTGCA ATTCACCTTC TTCAAGAATG TGGAAATCTC 421 TAATTTTGTC TGTGACCCAT CTCAACCTCT CAAGCTTGCC TGTTCTGACA GCATCATCGA 481 TAGCATGTTC ATATATTTCG ATAGTACTAT GTTTGGTTTT CTTCCCATTT CAGGGATCCT 541 TTTGTCTTAC TATAAAATTG TCCCCTCCAT TCTAGAACCC AGCTTTCTTG TACAAAGTGG 601 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 661 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 721 ACACCCGCTT ATAAACCTAC GGCGCACGCG TTAAGTCgac aatcaacctc tggattacaa 781 aatttgtgaa agatt