Construct: ORF TRCN0000488006
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020928.1_s317c1
- DNA Barcode:
- CAGGTGGGAGTGTATCAACACAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PTGER2 (5732)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488006
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5732 | PTGER2 | prostaglandin E receptor 2 | NM_000956.4 | 99.9% | 99.7% | 199T>C |
| 2 | mouse | 19217 | Ptger2 | prostaglandin E receptor 2 ... | NM_008964.4 | 82.9% | 85.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1146
- ORF length:
- 1074
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggcaat gcctccaatg actcccagtc tgaggactgc gagacgcgac 121 agtggcttcc cccaggcgaa agcccagcca tcagctccgt catgttctcg gccggggtgc 181 tggggaacct catagcactg gcgctgctgg cgcgccgctg gcggggggac gtggggtgca 241 gcgccggccg caggagctcc ctctccttgc tccacgtgct ggtgaccgag ctggtgttca 301 ccgacctgct cgggacctgc ctcatcagcc cagtggtact ggcttcgtac gcgcggaacc 361 agaccctggt ggcactggcg cccgagagcc gcgcgtgcac ctacttcgct ttcgccatga 421 ccttcttcag cctggccacg atgctcatgc tcttcgccat ggccctggag cgctacctct 481 cgatcgggca cccctacttc taccagcgcc gcgtctcgcg ctccgggggc ctggccgtgc 541 tgcctgtcat ctatgcagtc tccctgctct tctgctcgct gccgctgctg gactatgggc 601 agtacgtcca gtactgcccc gggacctggt gcttcatccg gcacgggcgg accgcttacc 661 tgcagctgta cgccaccctg ctgctgcttc tcattgtctc ggtgctcgcc tgcaacttca 721 gtgtcattct caacctcatc cgcatgcacc gccgaagccg gagaagccgc tgcggacctt 781 ccctgggcag tggccggggc ggccccgggg cccgcaggag aggggaaagg gtgtccatgg 841 cggaggagac ggaccacctc attctcctgg ctatcatgac catcaccTTC GCCGTCTGCT 901 CCTTGCCTTT CACGATTTTT GCATATATGA ATGAAACCTC TTCCCGAAAG GAAAAATGGG 961 ACCTCCAAGC TCTTAGGTTT TTATCAATTA ATTCAATAAT TGACCCTTGG GTCTTTGCCA 1021 TCCTTAGGCC TCCTGTTCTG AGACTAATGC GTTCAGTCCT CTGTTGTCGG ATTTCATTAA 1081 GAACACAAGA TGCAACACAA ACTTCCTGTT CTACACAGTC AGATGCCAGT AAACAGGCTG 1141 ACCTTTAGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1201 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1261 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACAGGTG GGAGTGTATC AACACAGTAC 1321 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt