Construct: ORF TRCN0000488026
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019931.1_s317c1
- DNA Barcode:
- AAAATCTACTGCCGCCCGAGCGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR39 (2863)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488026
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2863 | GPR39 | G protein-coupled receptor 39 | NM_001508.3 | 99.9% | 99.7% | 1359_1360insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1437
- ORF length:
- 1362
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggct tcacccagcc tcccgggcag tgactgctcc caaatcattg 121 atcacagtca tgtccccgag tttgaggtgg ccacctggat caaaatcacc cttattctgg 181 tgtacctgat catcttcgtg atgggccttc tggggaacag cgccaccatt cgggtcaccc 241 aggtgctgca gaagaaagga tacttgcaga aggaggtgac agaccacatg gtgagtttgg 301 cttgctcgga catcttggtg ttcctcatcg gcatgcccat ggagttctac agcatcatct 361 ggaatcccct gaccacgtcc agctacaccc tgtcctgcaa gctgcacact ttcctcttcg 421 aggcctgcag ctacgctacg ctgctgcacg tgctgacact cagctttgag cgctacatcg 481 ccatctgtca ccccttcagg tacaaggctg tgtcgggacc ttgccaggtg aagctgctga 541 ttggcttcgt ctgggtcacc tccgccctgg tggcactgcc cttgctgttt gccatgggta 601 ctgagtaccc cctggtgaac gtgcccagcc accggggtct cacttgcaac cgctccagca 661 cccgccacca cgagcagccc gagacctcca atatgtccat ctgtaccaac ctctccagcc 721 gctggaccgt gttccagtcc agcatcttcg gcgccttcgt ggtctacctc gtggtcctgc 781 tctccgtagc cttcatgtgc tggaacatga tgcaggtgct catgaaaagc cagaagggct 841 cgctggccgg gggcacgcgg cctccgcagc tgaggaagtc cgagagcgaa gagagcagga 901 ccgccaggag gcagaccatc atcttcctga ggctgattgt tgtgacattg gccgtatgct 961 ggatgcccaa ccagattcgg aggatcatgg ctgcggccaa acccaagcac gactggacga 1021 ggtcctactt ccgggcgtac atgatcctcc tccccttctc ggagacgttt ttctacctca 1081 gctcggtcat caacccgcTC CTGTACACGG TGTCCTCGCA GCAGTTTCGG CGGGTGTTCG 1141 TGCAGGTGCT GTGCTGCCGC CTGTCGCTGC AGCACGCCAA CCACGAGAAG CGCCTGCGCG 1201 TACATGCGCA CTCCACCACC GACAGCGCCC GCTTTGTGCA GCGCCCGTTG CTCTTCGCGT 1261 CCCGGCGCCA GTCCTCTGCA AGGAGAACTG AGAAGATTTT CTTAAGCACT TTTCAGAGCG 1321 AGGCCGAGCC CCAGTCTAAG TCCCAGTCAT TGAGTCTCGA GTCACTAGAG CCCAACTCAG 1381 GCGCGAAACC AGCCAATTCT GCTGCAGAGA ATGGTTTTCA GGAGCATGAA GTTGACCCAG 1441 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1501 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1561 TATCTTGTGG AAAGGACGAA AAATCTACTG CCGCCCGAGC GTGACGCGTT AAGTCgacaa 1621 tcaacctctg gattacaaaa tttgtgaaag att