Construct: ORF TRCN0000488072
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021363.1_s317c1
- DNA Barcode:
- TTGGACCCGGGCTATGCGGTACCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SLC6A15 (55117)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488072
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55117 | SLC6A15 | solute carrier family 6 mem... | NM_018057.6 | 99.7% | 99.6% | 786G>A;833A>T |
| 2 | human | 55117 | SLC6A15 | solute carrier family 6 mem... | XM_011538525.3 | 50.4% | 46.7% | (many diffs) |
| 3 | human | 55117 | SLC6A15 | solute carrier family 6 mem... | NM_182767.6 | 38.3% | 35.4% | (many diffs) |
| 4 | human | 55117 | SLC6A15 | solute carrier family 6 mem... | NM_001146335.3 | 23.6% | 20.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 939
- ORF length:
- 867
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcccaaa aatagcaagg tggtaaaaag agaattagat gatgatgtta 121 ctgagtctgt caaagacctt ctttccaatg aagacgcagc tgatgatgct tttaagacaa 181 gtgaactaat tgttgatggc caggaagaga aagatacaga tgttgaagaa ggatctgaag 241 tcgaagatga aagaccagct tggaacagta aactacaata catcctggcc caagttggat 301 tttctgtagg tttaggaaat gtgtggcgat ttccatacct atgtcagaag aatgggggcg 361 gtgcatatct tttaccatat ttaatactac ttatggtaat aggtattccc ctttttttct 421 tggaactctc tgtgggtcaa agaattcggc gaggcagcat tggtgtatgg aattacataa 481 gccctaaact gggcgggatt ggatttgcaa gttgtgtagt gtgctatttt gtagctctct 541 actacaacgt catcattggc tggagtttgt tttatttttc tcagtctttt cagcaacccc 601 tgccttggga tCAGTGTCCT TTGGTGAAAA ATGCTTCACA CACTTTTGTA GAACCAGAAT 661 GTGAACAAAG TTCTGCCACC ACCTATTACT GGTACAGGGA AGCACTGAAT ATTTCAAGTT 721 CCATTTCTGA AAGTGGGGGC TTAAACTGGA AGATGACCAT CTGCTTGTTG GCTGCCTGGG 781 TCATGGTTTG CTTGGCTATG ATCAAAGGCA TTCAGTCTTC TGGAAAAGTT AGTATGTTAG 841 AGCCCTTCCT CATTCTACTA ATCACCATTT CTGGATTCAT CCCTCTCTCA AATTCTGTTA 901 CAGTTTTCTG TGGGCAAATC ACACATAACA CTTCATTCTA GAACCCAGCT TTCTTGTACA 961 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1021 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1081 AGGACGATTG GACCCGGGCT ATGCGGTACC TACGCGTTAA GTCgacaatc aacctctgga 1141 ttacaaaatt tgtgaaagat t