Construct: ORF TRCN0000488081
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020030.1_s317c1
- DNA Barcode:
- CGATTAGCCCGACTGCCTGTGACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SRMS (6725)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488081
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6725 | SRMS | src-related kinase lacking ... | NM_080823.3 | 99.7% | 99.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1542
- ORF length:
- 1467
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggag ccgttcctca ggaggcggct ggccttcctg tccttcttct 121 gggacaagat ctggccggcg ggcggcgagc cggaccatgg cacccccggg tccctggacc 181 ccaacactga cccagtgccc acgctccccg ccgagccttg cagccccttc cctcagctct 241 tccttgcgct ctatgacttc acggcgcggt gtggcgggga gctgagtgtc cgccgcgggg 301 acaggctctg tgccctcgaa gaggggggcg gctacatctt cgcacgcagg ctttcgggcc 361 agcccagcgc cgggctcgtg cccatcaccc acgtggccaa ggcttctcct gagacgctct 421 cagaccaacc ctggtacttt agcggggtca gtcggaccca ggcacagcag ctgctcctct 481 ccccacccaa cgaaccaggg gccttcctca tccggcccag cgagagcagc ctcgggggct 541 actcactgtc agtccgggcc caggccaagg tctgccacta ccgggtctcc atggcagctg 601 atggcagcct ctacctgcag aagggacggc tctttcccgg cctggaggag ctgctcacct 661 attacaaggc caactggaag ctgatccaga accccctgct gcagccctgc atgccccaga 721 aggccccgag gcaggacgtg tgggagcggc cacactccga attcgccctt gggaggaagc 781 tgggtgaagg ctactttggg gaggtgtggg aaggcctgtg gctgggctcc ctgcccgtgg 841 cgatcaaggt catcaagtca gccaacatga agctcactga cctcgccaag gagatccaga 901 cactgaaggg cctgcggcac gagcggctca tccggctgca cgcagtgtgc tcgggcgggg 961 agcctgtgta catagtcacg gaactcatgc gcaaggggaa cctgcaggcc ttcctgggca 1021 cccccgaggg ccgggccctg cgtctgccgc cactcctggg ctttgcctgc caggtggctg 1081 agggcatgag ctacctggag gagcagcgcg ttgtgcaccg ggacttggcc gcccggaacg 1141 tgctcgtgga cgacggcctg gcctgcaagg tggctgactt cggcctggcc cggctgctca 1201 aggacgacat ctactccccg agcagcagct ccaagatccc ggtcaagtgg acagcgcctg 1261 aggcggccaa ttatcgtgtc ttctcccaga agtcagacgt ctggtccTTC GGCGTCCTGC 1321 TGCACGAGGT TTTCACCTAT GGCCAGTGTC CCTATGAAGG GATGACCAAC CACGAGACGC 1381 TGCAGCAGAT CATGCGAGGG TACCGGCTGC CGCGCCCGGC TGCCTGCCCG GCGGAGGTCT 1441 ACGTGCTCAT GCTGGAGTGC TGGAGGAGCA GCCCCGAGGA ACGGCCCTCC TTTGCCACGC 1501 TGCGGGAGAA GCTGCACGCC ATCCACAGAT GCCACCCCGA CCCAGCTTTC TTGTACAAAG 1561 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1621 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1681 ACGACGATTA GCCCGACTGC CTGTGACGAC GCGTTAAGTC gacaatcaac ctctggatta 1741 caaaatttgt gaaagatt