Construct: ORF TRCN0000488093
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020540.1_s317c1
- DNA Barcode:
- GGAAGTAAAGTCCGGTTTGGTTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SIK1 (150094)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488093
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 102724428 | SIK1B | salt inducible kinase 1B (p... | NM_001320643.3 | 100% | 100% | |
| 2 | human | 150094 | SIK1 | salt inducible kinase 1 | NM_173354.5 | 99.9% | 99.8% | 1844C>T;1848C>T |
| 3 | human | 102724428 | SIK1B | salt inducible kinase 1B (p... | XM_017028208.1 | 93.7% | 93.7% | 967_968ins147 |
| 4 | human | 150094 | SIK1 | salt inducible kinase 1 | XM_011529474.2 | 93.6% | 93.6% | 967_968ins147;1697C>T;1701C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2421
- ORF length:
- 2349
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggttatc atgtcggagt tcagcgcgga ccccgcgggc cagggtcagg 121 gccagcagaa gcccctccgg gtgggttttt acgacatcga gcggaccctg ggcaaaggca 181 acttcgcggt ggtgaagctg gcgcggcatc gagtcaccaa aacgcaggtt gcaataaaaa 241 taattgataa aacacgatta gattcaagca atttggagaa aatctatcgt gaggttcagc 301 tgatgaagct tctgaaccat ccacacatca taaagcttta ccaggttatg gaaacaaagg 361 acatgcttta catcgtcact gaatttgcta aaaatggaga aatgtttgat tatttgactt 421 ccaacgggca cctgagtgag aacgaggcgc ggaagaagtt ctggcaaatc ctgtcggccg 481 tggagtactg tcacgaccat cacatcgtcc accgggacct caagaccgag aacctcctgc 541 tggatggcaa catggacatc aagctggcag attttggatt tgggaatttc tacaagtcag 601 gagagcctct gtccacgtgg tgtgggagcc ccccgtatgc cgccccggaa gtctttgagg 661 ggaaggagta tgaaggcccc cagctggaca tctggagcct gggcgtggtg ctgtacgtcc 721 tggtctgcgg ttctctcccc ttcgatgggc ctaacctgcc gacgctgaga cagcgggtgc 781 tggagggccg cttccgcatc cccttcttca tgtctcaaga ctgtgagagc ctgatccgcc 841 gcatgctggt ggtggacccc gccaggcgca tcaccatcgc ccagatccgg cagcaccggt 901 ggatgcgggc tgagccctgc ttgccgggac ccgcctgccc cgccttctcc gcacacagct 961 acacctccaa cctgggcgac tacgatgagc aggcgctggg tatcatgcag accctgggcg 1021 tggaccggca gaggacggtg gagtcactgc aaaacagcag ctataaccac tttgctgcca 1081 tttattacct cctccttgag cggctcaagg agtatcggaa tgcccagtgc gcccgccccg 1141 ggcctgccag gcagccgcgg cctcggagct cggacctcag tggtttggag gtgcctcagg 1201 aaggtctttc caccgaccct ttccgacctg ccttgctgtg cccgcagccg cagaccttgg 1261 tgcagtccgt cctccaggcc gagatggact gtgagctcca gagctcgctg cagtggccct 1321 tgttcttccc ggtggatgcc agctgcagcg gagtgttccg gccccggccc gtgtccccaa 1381 gcagcctgct ggacacagcc atcagtgagg aggccaggca ggggccgggc ctagaggagg 1441 agcaggacac gcaggagtcc ctgcccagca gcacgggccg gaggcacacc ctggccgagg 1501 tctccacccg cctctcccca ctcaccgcgc catgtatagt cgtctccccc tccaccacgg 1561 caagtcctgc agagggaacc agctctgaca gttgtctgac cttctctgcg agcaaaagcc 1621 ccgcggggct cagtggcacc ccggccactc aggggctgct gggcgcctgc tccccggtca 1681 ggctggcctc gcccttcctg gggtcgcagt ccgccacccc agtgctgcag gctcaggggg 1741 gcttgggagg agctgttctg ctccctgtca gcttccagga gggacggcgg gcgtcggaca 1801 cctcactgac tcaagggctg aaggcctttc ggcagcagct gaggaagacc acgcggacca 1861 aagggtttct gggactgaac aaaatcaagg ggctggctcg ccaggtgtgc caggtccctg 1921 ccagccgggc cagcaggggc ggcctgagcc ccttccacgc ccctgcacag agcccaggcc 1981 tgcacggcgg cgcagccggc agccgggagg gctggagcct gctggaggag gtgctagagc 2041 agcagaggct gctccagtta cagcaccacc cggccgctgc acccggctgc tcccaggccc 2101 cCCAGCCGGC CCCTGCCCCG TTTGTGATCG CCCCCTGTGA TGGCCCTGGG GCTGCCCCGC 2161 TCCCCAGCAC CCTCCTCACG TCGGGGCTCC CGCTGCTGCC GCCCCCACTC CTGCAGACCG 2221 GCGCGTCCCC GGTGGCCTCA GCGGCGCAGC TCCTGGACAC ACACCTGCAC ATTGGCACCG 2281 GCCCCACCGC CCTCCCCGCT GTGCCCCCAC CACGCCTGGC CAGGCTGGCC CCAGGTTGTG 2341 AGCCCCTGGG GCTGCTGCAG GGGGACTGTG AGATGGAGGA CCTGATGCCC TGCTCCCTAG 2401 GCACGTTTGT CCTGGTGCAG TAGGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2461 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2521 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG GAAGTAAAGT 2581 CCGGTTTGGT TTAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2641 att