Construct: ORF TRCN0000488109
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020410.1_s317c1
- DNA Barcode:
- TATTAAGGGAGACCACCATAGAGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CDK2 (1017)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488109
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1017 | CDK2 | cyclin dependent kinase 2 | NM_001798.5 | 100% | 100% | |
| 2 | human | 1017 | CDK2 | cyclin dependent kinase 2 | NM_052827.4 | 88.5% | 88.5% | 485_486ins102 |
| 3 | human | 1017 | CDK2 | cyclin dependent kinase 2 | XM_011537732.2 | 86.1% | 86.1% | 589_732del |
| 4 | human | 1017 | CDK2 | cyclin dependent kinase 2 | NM_001290230.2 | 79.8% | 79.8% | 116_117ins78;407_408ins102 |
| 5 | mouse | 12566 | Cdk2 | cyclin-dependent kinase 2 | NM_016756.4 | 92.5% | 98.9% | (many diffs) |
| 6 | mouse | 12566 | Cdk2 | cyclin-dependent kinase 2 | NM_183417.3 | 79.6% | 85.2% | (many diffs) |
| 7 | mouse | 12566 | Cdk2 | cyclin-dependent kinase 2 | XM_006513162.2 | 55.7% | 59.5% | (many diffs) |
| 8 | mouse | 12566 | Cdk2 | cyclin-dependent kinase 2 | XM_011243321.2 | 55.7% | 59.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 84
- ORF end:
- 978
- ORF length:
- 894
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggcccccttc accatggaga acttccaaaa ggtggaaaag atcggagagg 121 gcacgtacgg agttgtgtac aaagccagaa acaagttgac gggagaggtg gtggcgctta 181 agaaaatccg cctggacact gagactgagg gtgtgcccag tactgccatc cgagagatct 241 ctctgcttaa ggagcttaac catcctaata ttgtcaagct gctggatgtc attcacacag 301 aaaataaact ctacctggtt tttgaatttc tgcaccaaga tctcaagaaa ttcatggatg 361 cctctgctct cactggcatt cctcttcccc tcatcaagag ctatctgttc cagctgctcc 421 agggcctagc tttctgccat tctcatcggg tcctccaccg agaccttaaa cctcagaatc 481 tgcttattaa cacagagggg gccatcaagc tagcagactt tggactagcc agagcttttg 541 gagtccctgt tcgtacttac acccatgagg tggtgaccct gtggtaccga gctcctgaaa 601 tcctcctggg ctgcaaatat tattccacag ctgtggacat ctggagcctg ggctgcatct 661 ttgctgagat ggtgactcgc cgggCCCTAT TCCCTGGAGA TTCTGAGATT GACCAGCTCT 721 TCCGGATCTT TCGGACTCTG GGGACCCCAG ATGAGGTGGT GTGGCCAGGA GTTACTTCTA 781 TGCCTGATTA CAAGCCAAGT TTCCCCAAGT GGGCCCGGCA AGATTTTAGT AAAGTTGTAC 841 CTCCCCTGGA TGAAGATGGA CGGAGCTTGT TATCGCAAAT GCTGCACTAC GACCCTAACA 901 AGCGGATTTC GGCCAAGGCA GCCCTGGCTC ACCCTTTCTT CCAGGATGTG ACCAAGCCAG 961 TACCCCATCT TCGACTCTGA AAGCTTAAGG GTGGGCGCGC CGACCCAGCT TTCTTGTACA 1021 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1081 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1141 AGGACGATAT TAAGGGAGAC CACCATAGAG CACGCGTTAA GTCgacaatc aacctctgga 1201 ttacaaaatt tgtgaaagat t