Construct: ORF TRCN0000488133
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021779.1_s317c1
- DNA Barcode:
- GGTTTACGCCTATCCCCCCTTGAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PLCG2 (5336)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488133
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5336 | PLCG2 | phospholipase C gamma 2 | NM_002661.5 | 99.8% | 99.7% | (many diffs) |
| 2 | mouse | 234779 | Plcg2 | phospholipase C, gamma 2 | NM_172285.2 | 86.4% | 93.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3867
- ORF length:
- 3795
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtccacc acggtcaatg tagattccct tgcggaatat gagaagagcc 121 agatcaagag agccctggag ctggggacgg tgatgactgt gttcagcttc cgcaagtcca 181 cccccgagcg gagaaccgtc caggtgatca tggagacgcg gcaggtggcc tggagcaaga 241 ccgccgacaa gatcgagggc ttcttggata tcatggaaat aaaagaaatc cgcccaggga 301 agaactccaa agatttcgag cgagcaaaag cagttcgcca gaaagaagac tgctgcttca 361 ccatcctata tggcactcag ttcgtcctca gcacgctcag cttggcagct gactctaaag 421 aggatgcagt taactggctc tctggcttga aaatcttaca ccaggaagcg atgaatgcgt 481 ccacgcccac cattatcgag agttggctga gaaagcagat atattctgtg gatcaaacca 541 gaagaaacag catcagtctc cgagagttga agaccatctt gcccctgatc aactttaaag 601 tgagcagtgc caagttcctt aaagataagt ttgtggaaat aggagcacac aaagatgagc 661 tcagctttga acagttccat ctcttctata aaaaacttat gtttgaacag caaaaatcga 721 ttctcgatga attcaaaaag gattcgtccg tgttcatcct ggggaacact gacaggccgg 781 atgcctctgc tgtttacctg catgacttcc agaggtttct catacatgaa cagcaggagc 841 attgggctca ggatctgaac aaagtccgtg agcggatgac aaagttcatt gatgacacca 901 tgcgtgaaac tgctgagcct ttcttgtttg tggatgagtt cctcacgtac ctgttttcac 961 gagaaaacag catctgggat gagaagtatg acgcggtgga catgcaggac atgaacaacc 1021 ccctgtctca ttactggatc tcctcgtcac ataacacgta ccttacaggt gaccagctgc 1081 ggagcgagtc gtccccagaa gcttacatcc gctgcctgcg catgggctgt cgctgcattg 1141 aactggactg ctgggacggg cccgatggga agccggtcat ctaccatggc tggacgcgga 1201 ctaccaagat caagtttgac gacgtcgtgc aggccatcaa agaccacgcc tttgttacgt 1261 cgagcttccc agtgatcctg tccatcgagg agcactgcag cgtggagcaa cagcgtcaca 1321 tggccaaggc cttcaaggaa gtatttggcg acctgctgtt gacgaagccc acggaggcca 1381 gtgctgacca gctgccctcg cccagccagc tgcgggagaa gatcatcatc aagcataaga 1441 agctgggccc ccgaggcgat gtggatgtca acatggagga caagaaggac gaacacaagc 1501 aacaggggga gctgtacatg tgggattcca ttgaccagaa atggactcgg cactactgcg 1561 ccattgccga tgccaagctg tccttcagtg atgacattga acagactatg gaggaggaag 1621 tgccccagga tataccccct acagaactac attttgggga gaaatggttc cacaagaagg 1681 tggagaagag gacgagtgcc gagaagttgc tgcaggaata ctgcatggag acggggggca 1741 aggatggcac cttcctggtt cgggagagcg agaccttccc caatgactac accctgtcct 1801 tctggcggtc aggccgggtc cagcactgcc ggatccgctc caccatggag ggcgggaccc 1861 tgaaatacta cttgactgac aacctcacct tcagcagcat ctatgccctc atccagcact 1921 accgcgagac gcacctgcgc tgcgccgagt tcgagctgcg gctcacggac cctgtgccca 1981 accccaaccc ccacgagtcc aagccgtggt actatgacag cctgagccgc ggagaggcag 2041 aggacatgct gatgaggatt ccccgggacg gggccttcct gatccggaag cgagagggga 2101 gcgactccta tgccatcacc ttcagggcta ggggcaaggt aaagcattgt cgcatcaacc 2161 gggacggccg gcactttgtg ctggggacct ccgcctattt tgagagtctg gtggagctcg 2221 tcagttacta cgagaagcat tcactctacc gaaagatgag actgcgctac cccgtgaccc 2281 ccgagctcct ggagcgctac aatatggaaa gagatataaa ctccctctac gacgtcagca 2341 gaatgtttgt ggatcccagt gaaatcaatc cgtccatgcc tcagagaacc gtgaaagctc 2401 tgtatgacta caaagccaag cgaagcgatg agctgagctt ctgccgtggt gccctcatcc 2461 acaatgtctc caaggagccc gggggctggt ggaaaggaga ctatggaacc aggatccagc 2521 agtacttccc atccaactac gtcgaggaca tctcaactgc agacttcgag gagctagaaa 2581 agcagattat tgaagacaat cccttagggt ctctttgcag aggaatattg gacctcaata 2641 cctataacgt cgtgaaagcc cctcagggaa aaaaccagaa gtcctttgtc ttcatcctgg 2701 agcccaagca gcagggcgat cctccggtgg agtttgccac agacagggtg gaggagctct 2761 ttgagtggtt tcagagcatc cgagagatca cctggaagat tgacaccaag gagaacaaca 2821 tgaagtactg ggagaagaac cagtccatcg ccatcgagct ctctgacctg gttgtctact 2881 gcaaaccaac cagcaaaacc aaggacaact tagaaaatcc tgacttccga gaaatccgct 2941 cctttgtgga gacgaaggct gacagcatca tcagacagaa gcccgtcgac ctcctgaagt 3001 acaatcaaaa gggcctgacc cgcgtctacc caaagggaca aagagttgac tcttcaaact 3061 acgacccctt ccgcctctgg ctgtgcggtt ctcagatggt ggcactcaat ttccagacgg 3121 cagataagta catgcagatg aatcacgcat tgttttctct caacgggcgc acgggctacg 3181 ttctgcagcc tgagagcatg aggacagaga aatatgaccc gatgccaccc gagtcccaga 3241 ggaagatcct gatgacgctg acagtcaagg ttctcggtgc tcgccatctc cccaaacttg 3301 gacgaagtat tgcctgtccc tttgtagaag tggagatctg tggagccgag tatgacaaca 3361 acaagttcaa gacgacggtt gtgaatgata atggcctcag ccctatctgg gctccaacac 3421 aggagaaggt gacatttgaa atttatgacc caaacctggc atttctgcgc tttgtggttt 3481 atgaagaaga tatgttcagc gatcccaact ttcttgctca tgccacttac cccattaaag 3541 cagtcaaatc aggattcagg tccgttcctc TGAAGAATGG GTACAGCGAG GACATAGAGC 3601 TGGCTTCCCT CCTGGTTTTC TGTGAGATGC GGCCAGTCCT GGAGAGCGAA GAGGAACTTT 3661 TCTCCTCCTG TCGCCAGCTG AGGAGGCGGC AAGAAGAACT GAACAACCAG CTCTTTCTGT 3721 TTGACACACA CCAGAACTTG CGCAATGCCA ACCGGGATGC CCTGGTTAAA GAGTTCAGTG 3781 TTAATGAGAA CCAGCTCCAG CTGTACCAGG AGAAATGCAA CAAGAGGTTA AGAGAGAAGA 3841 GAGTCAGCAA CAGCAAGTTT TACTCATAGA ACCCAGCTTT CTTGTACAAA GTGGTTGATA 3901 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 3961 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGTTT 4021 ACGCCTATCC CCCCTTGAGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 4081 tgaaagatt