Construct: ORF TRCN0000488148
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019909.1_s317c1
- DNA Barcode:
- GATTATATTTATTCCCCATTTCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GCLC (2729)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488148
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2729 | GCLC | glutamate-cysteine ligase c... | NM_001498.4 | 99.9% | 99.8% | 1911_1912insG |
| 2 | human | 2729 | GCLC | glutamate-cysteine ligase c... | NM_001197115.2 | 93.9% | 93.8% | 443_444ins114;1797_1798insG |
| 3 | human | 2729 | GCLC | glutamate-cysteine ligase c... | XM_017010749.1 | 62.7% | 62.6% | 0_1ins711;1200_1201insG |
| 4 | mouse | 14629 | Gclc | glutamate-cysteine ligase, ... | NM_010295.2 | 88.4% | 94.3% | (many diffs) |
| 5 | mouse | 14629 | Gclc | glutamate-cysteine ligase, ... | XM_006510812.1 | 80.7% | 86.2% | (many diffs) |
| 6 | mouse | 14629 | Gclc | glutamate-cysteine ligase, ... | XR_001778796.1 | 46.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1983
- ORF length:
- 1914
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggggctgctg tcccagggct cgccgctgag ctgggaggaa accaagcgcc 121 atgccgacca cgtgcggcgg cacgggatcc tccagttcct gcacatctac cacgccgtca 181 aggaccggca caaggacgtt ctcaagtggg gcgatgaggt ggaatacatg ttggtatctt 241 ttgatcatga aaataaaaaa gtccggttgg tcctgtctgg ggagaaagtt cttgaaactc 301 tgcaagagaa gggggaaagg acaaacccaa accatcctac cctttggaga ccagagtatg 361 ggagttacat gattgaaggg acaccaggac agccctacgg aggaacaatg tccgagttca 421 atacagttga ggccaacatg cgaaaacgcc ggaaggaggc tacttctata ttagaagaaa 481 atcaggctct ttgcacaata acttcatttc ccagattagg ctgtcctggg ttcacactgc 541 ccgaggtcaa acccaaccca gtggaaggag gagcttccaa gtccctcttc tttccagatg 601 aagcaataaa caagcaccct cgcttcagta ccttaacaag aaatatccga cataggagag 661 gagaaaaggt tgtcatcaat gtaccaatat ttaaggacaa gaatacacca tctccattta 721 tagaaacatt tactgaggat gatgaagctt caagggcttc taagccggat catatttaca 781 tggatgccat gggatttgga atgggcaatt gctgtctcca ggtgacattc caagcctgca 841 gtatatctga ggccagatac ctttatgatc agttggctac tatctgtcca attgttatgg 901 ctttgagtgc tgcatctccc ttttaccgag gctatgtgtc agacattgat tgtcgctggg 961 gagtgatttc tgcatctgta gatgatagaa ctcgggagga gcgaggactg gagccattga 1021 agaacaataa ctataggatc agtaaatccc gatatgactc aatagacagc tatttatcta 1081 agtgtggtga gaaatataat gacatcgact tgacgataga taaagagatc tacgaacagc 1141 tgttgcagga aggcattgat catctcctgg cccagcatgt tgctcatctc tttattagag 1201 acccactgac actgtttgaa gagaaaatac acctggatga tgctaatgag tctgaccatt 1261 ttgagaatat tcagtccaca aattggcaga caatgagatt taagccccct cctccaaact 1321 cagacattgg atggagagta gaatttcgac ccatggaggt gcaattaaca gactttgaga 1381 actctgccta tgtggtgttt gtggtactgc tcaccagagt gatcctttcc tacaaattgg 1441 attttctcat tccactgtca aaggttgatg agaacatgaa ggtagcacag aaaagagatg 1501 ctgtcttgca gggaatgttt tatttcagga aagatatttg caaaggtggc aatgcagtgg 1561 tggatggttg tggcaaggcc cagaacagca cggagctcgc tgcagaggag tacaccctca 1621 tgagcataga caccatcatc aatgggaagg aaggtgtgtt tcctggactg atcccaattc 1681 tgaactctta ccttGAAAAC ATGGAAGTGG ATGTGGACAC CAGATGTAGT ATTCTGAACT 1741 ACCTAAAGCT AATTAAGAAG AGAGCATCTG GAGAACTAAT GACAGTTGCC AGATGGATGA 1801 GGGAGTTTAT CGCAAACCAT CCTGACTACA AGCAAGACAG TGTCATAACT GATGAAATGA 1861 ATTATAGCCT TATTTTGAAG TGTAACCAAA TTGCAAATGA ATTATGTGAA TGCCCAGAGT 1921 TACTTGGATC AGCATTTAGG AAAGTAAAAT ATAGTGGAAG TAAAACTGAC TCATCCAACG 1981 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2041 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2101 TTTATATATC TTGTGGAAAG GACGAGATTA TATTTATTCC CCATTTCTAA CGCGTTAAGT 2161 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt