Construct: ORF TRCN0000488162

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020366.1_s317c1
DNA Barcode:
AGCCAGTTCCAAAAACTTGGTTAG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
TSSK2 (23617)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488162
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23617 TSSK2 testis specific serine kina... NM_053006.5 99.9% 99.7% 911G>A
2 mouse 22115 Tssk2 testis-specific serine kina... NM_009436.2 89.6% 91.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1146
ORF length:
1074
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggacgat gccacagtcc taaggaagaa gggttacatc gtaggcatca
121atcttggcaa gggttcctac gcaaaagtca aatctgccta ctctgagcgc ctcaagttca
181atgtggctgt caagatcatc gaccgcaaga aaacacctac tgactttgtg gagagattcc
241ttcctcggga gatggacatc ctggcaactg tcaaccacgg ctccatcatc aagacttacg
301agatctttga gacctctgac ggacggatct acatcatcat ggagcttggc gtccagggcg
361acctcctcga gttcatcaag tgccagggag ccctgcatga ggacgtggca cgcaagatgt
421tccgacagct ctcctccgcc gtcaagtact gccacgacct ggacatcgtc caccgggacc
481tcaagtgcga gaaccttctc ctcgacaagg acttcaacat caagctgtct gactttggct
541tctccaagcg ctgcctgcgg gacagcaatg ggcgcatcat cctcagcaag accttctgcg
601ggtcggcagc atatgcagcc cccgaggtgc tgcagagcat cccctaccag cccaaggtgt
661atgacatctg gagcctgggc gtgatcctgt acatcatggt ctgcggctcc atgccctatg
721acgactccga catcaggaag atgctgcgta tccagaagga gcaccgtgtg gacttcccgc
781gctccaagaa cctgacctgc gagtgcaagg acctcatcta ccgcatgctg cagcccgacg
841tcagccagcg gctccacatc gatgagatcc TCAGCCACTC GTGGCTGCAG CCCCCCAAGC
901CCAAAGCCAC GTCTTCTGCC TCCTTCAAGA GGGAGGGGGA GGGCAAGTAC CGCGCTGAGT
961GCAAACTGGA CACCAAGACA GACTTGAGGC CCGACCACCG GCCCGACCAC AAGCTTGGAG
1021CCAAAACCCA GCACCGGCTG CTGGTGGTGC CCGAGAACGA GAACAGGATG GAGGACAGGC
1081TGGCCGAGAC CTCCAGGGCC AAAGACCATC ACATCTCCGG AGCTGAGGTG GGGAAAGCAA
1141GCACCTAGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1201CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1261TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGCCAG TTCCAAAAAC TTGGTTAGAC
1321GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)

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