Construct: ORF TRCN0000488182
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019710.1_s317c1
- DNA Barcode:
- TGCGGGCTATTCAGCGCGTGCGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPHB3 (2049)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488182
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2049 | EPHB3 | EPH receptor B3 | NM_004443.4 | 99.9% | 99.8% | 2994_2995insG |
| 2 | mouse | 13845 | Ephb3 | Eph receptor B3 | NM_010143.1 | 88.1% | 96% | (many diffs) |
| 3 | mouse | 13845 | Ephb3 | Eph receptor B3 | XM_006521788.1 | 83.5% | 91% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 3072
- ORF length:
- 2997
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggcc agagcccgcc cgccgccgcc gccgtcgccg ccgccggggc 121 ttctgccgct gctccctccg ctgctgctgc tgccgctgct gctgctgccc gccggctgcc 181 gggcgctgga agagaccctc atggacacaa aatgggtaac atctgagttg gcgtggacat 241 ctcatccaga aagtgggtgg gaagaggtga gtggctacga tgaggccatg aatcccatcc 301 gcacatacca ggtgtgtaat gtgcgcgagt caagccagaa caactggctt cgcacggggt 361 tcatctggcg gcgggatgtg cagcgggtct acgtggagct caagttcact gtgcgtgact 421 gcaacagcat ccccaacatc cccggctcct gcaaggagac cttcaacctc ttctactacg 481 aggctgacag cgatgtggcc tcagcctcct cccccttctg gatggagaac ccctacgtga 541 aagtggacac cattgcaccc gatgagagct tctcgcggct ggatgccggc cgtgtcaaca 601 ccaaggtgcg cagctttggg ccactttcca aggctggctt ctacctggcc ttccaggacc 661 agggcgcctg catgtcgctc atctccgtgc gcgccttcta caagaagtgt gcatccacca 721 ccgcaggctt cgcactcttc cccgagaccc tcactggggc ggagcccacc tcgctggtca 781 ttgctcctgg cacctgcatc cctaacgccg tggaggtgtc ggtgccactc aagctctact 841 gcaacggcga tggggagtgg atggtgcctg tgggtgcctg cacctgtgcc accggccatg 901 agccagctgc caaggagtcc cagtgccgcc cctgtccccc tgggagctac aaggcgaagc 961 agggagaggg gccctgcctc ccatgtcccc ccaacagccg taccacctcc ccagccgcca 1021 gcatctgcac ctgccacaat aacttctacc gtgcagactc ggactctgcg gacagtgcct 1081 gtaccaccgt gccatctcca ccccgaggtg tgatctccaa tgtgaatgaa acctcactga 1141 tcctcgagtg gagtgagccc cgggacctgg gtggccggga tgacctcctg tacaatgtca 1201 tctgcaagaa gtgccatggg gctggagggg cctcagcctg ctcacgctgt gatgacaacg 1261 tggagtttgt gcctcggcag ctgggcctga cggagcgccg ggtccacatc agccatctgc 1321 tggcccacac gcgctacacc tttgaggtgc aggcggtcaa cggtgtctcg ggcaagagcc 1381 ctctgccgcc tcgttatgcg gccgtgaata tcaccacaaa ccaggctgcc ccgtctgaag 1441 tgcccacact acgcctgcac agcagctcag gcagcagcct caccctatcc tgggcacccc 1501 cagagcggcc caacggagtc atcctggact acgagatgaa gtactttgag aagagcgagg 1561 gcatcgcctc cacagtgacc agccagatga actccgtgca gctggacggg cttcggcctg 1621 acgcccgcta tgtggtccag gtccgtgccc gcacagtagc tggctatggg cagtacagcc 1681 gccctgccga gtttgagacc acaagtgaga gaggctctgg ggcccagcag ctccaggagc 1741 agcttcccct catcgtgggc tccgctacag ctgggcttgt cttcgtggtg gctgtcgtgg 1801 tcatcgctat cgtctgcctc aggaagcagc gacacggctc tgattcggag tacacggaga 1861 agctgcagca gtacattgct cctggaatga aggtttatat tgaccctttt acctacgagg 1921 accctaatga ggctgttcgg gagtttgcca aggagatcga cgtgtcctgc gtcaagatcg 1981 aggaggtgat cggagctggg gaatttgggg aagtgtgccg tggtcgactg aaacagcctg 2041 gccgccgaga ggtgtttgtg gccatcaaga cgctgaaggt gggctacacc gagaggcagc 2101 ggcgggactt cctaagcgag gcctccatca tgggtcagtt tgatcacccc aatataatcc 2161 ggctcgaggg cgtggtcacc aaaagtcggc cagttatgat cctcactgag ttcatggaaa 2221 actgcgccct ggactccttc ctccggctca acgatgggca gttcacggtc atccagctgg 2281 tgggcatgtt gcggggcatt gctgccggca tgaagtacct gtccgagatg aactatgtgc 2341 accgcgacct ggctgctcgc aacatccttg tcaacagcaa cctggtctgc aaagtctcag 2401 actttggcct ctcccgcttc ctggaggatg acccctccga tcctacctac accagttccc 2461 tgggcgggaa gatccccatc cgctggactg ccccagaggc catagcctat cggaagttca 2521 cttctgctag tgatgtctgg agctacggaa ttgtcatgtg ggaggtcatg agctatggag 2581 agcgacccta ctgggacatg agcaaccagg atgtcatcaa tgccgtggag caggattacc 2641 ggctgccacc acccatggac tgtcccacag cactgcacca gctcatgctg gactgctggg 2701 tgcgggaccg gaacctcagg cccaaattct cccagattgt caataccctg gacaagctca 2761 tccgcaatgc tgccagcctc aaggtcattg ccagcgctca gtctggcatg tcacagcccc 2821 TCCTGGACCG CACGGTCCCA GATTACACAA CCTTCACGAC AGTTGGTGAT TGGCTGGATG 2881 CCATCAAGAT GGGGCGGTAC AAGGAGAGCT TCGTCAGTGC GGGGTTTGCA TCTTTTGACC 2941 TGGTGGCCCA GATGACGGCA GAAGACCTGC TCCGTATTGG GGTCACCCTG GCCGGCCACC 3001 AGAAGAAGAT CCTGAGCAGT ATCCAGGACA TGCGGCTGCA GATGAACCAG ACGCTGCCTG 3061 TGCAGGTCGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 3121 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 3181 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATGCGGG CTATTCAGCG CGTGCGTGAC 3241 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt