Construct: ORF TRCN0000488185
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021940.1_s317c1
- DNA Barcode:
- CACGACACCGGTTTTAATGATCAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TESK2 (10420)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488185
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10420 | TESK2 | testis associated actin rem... | NM_007170.3 | 100% | 100% | |
| 2 | human | 10420 | TESK2 | testis associated actin rem... | NM_001320800.2 | 85.4% | 85.4% | 0_1ins249 |
| 3 | mouse | 230661 | Tesk2 | testis-specific kinase 2 | NM_146151.4 | 86.7% | 87.7% | (many diffs) |
| 4 | mouse | 230661 | Tesk2 | testis-specific kinase 2 | XM_006503008.3 | 68.8% | 69.1% | (many diffs) |
| 5 | mouse | 230661 | Tesk2 | testis-specific kinase 2 | XR_001784124.1 | 44.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1782
- ORF length:
- 1713
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggatcggagc aaacggaatt caattgcagg atttcctcca cgtgtggagc 121 gtcttgaaga gtttgaagga ggtggtggag gagaaggaaa tgtgagccag gtgggaagag 181 tttggccatc ttcgtatcga gctcttataa gtgccttttc cagactgacg cgtttggatg 241 atttcacctg tgaaaaaata gggtctggct tcttttctga agtgttcaag gtacgacacc 301 gagcttctgg tcaggtgatg gctcttaaga tgaacacatt gagcagtaac cgggcaaaca 361 tgctgaaaga agtacagctc atgaatagac tctcccatcc caacatcctt aggttcatgg 421 gtgtatgtgt tcatcaagga caattgcatg cacttacaga gtatatcaac tccgggaacc 481 tggaacagtt gctagacagt aacctgcatt tgccttggac tgtgagggta aaactggcct 541 atgacatagc agtgggcctc agctaccttc acttcaaagg catttttcat cgggacctca 601 catctaagaa ctgcctgata aagagggatg agaatggtta ctctgcagtg gtagctgact 661 ttggcctggc tgagaagatc cccgatgtca gcatggggag tgagaagctg gccgtggtgg 721 gttccccatt ctggatggca cctgaggttc tccgagatga gccctataat gaaaaggcag 781 atgtgttctc ttatggtatc atcctctgcg agatcatcgc ccgcatccag gccgatccgg 841 actatcttcc ccgcacagag aatttcgggc tggactatga tgctttccag cacatggtgg 901 gagactgtcc cccagatttt ctgcaactta ctttcaactg ctgtaacatg gatcccaaac 961 tgcgcccatc ttttgtggag attgggaaga ccctggagga aattctgagc cgcctacagg 1021 aagaagagca ggagagggat aggaagctgc agcccacagc caggggactc ttggagaaag 1081 cacctggggt gaagcgacta agctcactgg atgacaagat cccccacaag tcaccatgcc 1141 caagacgtac catctggctg tctcgaagcc agtcagatat cttttcccgt aagcccccac 1201 gtacagtgag tgtcttggac ccatactacc ggccacgaga tggtgctgcc cgcaccccca 1261 aagtcaaccc ttttagtgct cgccaggacc tcatgggggg caagatcaag ttttttgacc 1321 tgcccagcaa gtctgtcatc tctctggtat ttgacctgga tgcaccaggg cccggaacta 1381 tgcccctggc tgactggcag gagcccctgg ccccacctat tcgccggtgg cgttccttgc 1441 ctggttcgcc TGAGTTCTTG CATCAAGAGG CTTGTCCATT TGTGGGCCGG GAAGAATCGC 1501 TATCTGATGG GCCCCCACCA CGCCTAAGTA GTCTCAAGTA CAGAGTTAAA GAGATCCCAC 1561 CATTCCGGGC ATCTGCCCTA CCAGCTGCTC AAGCCCATGA GGCTATGGAC TGCTCCATTC 1621 TCCAGGAAGA AAATGGTTTT GGGTCCAGGC CCCAGGGGAC CAGTCCATGC CCTGCGGGTG 1681 CTTCTGAGGA GATGGAGGTA GAAGAAAGGC CAGCAGGCTC AACTCCAGCC ACCTTCTCCA 1741 CCTCAGGCAT AGGCCTGCAA ACCCAGGGAA AGCAGGATGG GTGAGACCCA GCTTTCTTGT 1801 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1861 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1921 GAAAGGACGA CACGACACCG GTTTTAATGA TCATACGCGT TAAGTCgaca atcaacctct 1981 ggattacaaa atttgtgaaa gatt