Construct: ORF TRCN0000488191
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021291.1_s317c1
- DNA Barcode:
- CGATCCTACGTGCCACGCCGATTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NLN (57486)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488191
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57486 | NLN | neurolysin | XM_005248559.3 | 80.1% | 68.2% | (many diffs) |
| 2 | human | 57486 | NLN | neurolysin | NM_020726.5 | 79.6% | 70.6% | (many diffs) |
| 3 | human | 57486 | NLN | neurolysin | XM_017009673.1 | 76.9% | 65.1% | (many diffs) |
| 4 | human | 57486 | NLN | neurolysin | XM_006714661.2 | 76.4% | 67.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1893
- ORF length:
- 1821
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgatcgcc cggtgccttt tggctgtgcg aagcctccgc agagttggtg 121 gttccaggat tttactcaga atgacgttag gaagagaagt gatgtctcct cttcaggcaa 181 tgtcttccta tactgtggct ggcagaaatg ttttaagatg ggatctttca ccagagcaaa 241 ttaaaacaag aactgaggag ctcattgtgc agaccaaaca ggtgtacgat gctgttggaa 301 tgctcggtat tgaggaagta acttacgaga actgtctgca ggcactggca gatgtagaag 361 taaagtatat agtggaaagg accatgctag actttcccca gcatgtatcc tctgacaaag 421 aagtacgagc agcaagtaca gaagcagaca aaagactttc tcgttttgat attgagatga 481 gcatgagagg agatatattt gagagaattg ttcatttaca ggaaacctgt gatctgggga 541 agataaaacc tgaggccaga cgatacttgg aaaagtcaat taaaatgggg aaaagaaatg 601 ggctccatct tcctgaacaa gtacagaatg aaatcaaatc aatgaagaaa agaatgagtg 661 agctatgtat tgattttaac aaaaacctca atgaggatga taccttcctt gtattttcca 721 aggctgaact tggtgctctt cctcatgatt tcattgacag tttagaaaag acagatgatg 781 acaagtataa aattacctta aaatatccac actatttccc tgtcatgaag aaatgttgta 841 tccctgaaac cagaagaagg atggaaatgg cttttaatac aaggtgcaaa gaggaaaaca 901 ccataatttt gcagcagcta ctcccactgc gaaccaaggt ggccaaacta ctcggttata 961 gcacacatgc tgacttcgtc ctcgaaatga acactgcaaa gagcacaagc cgcgtaacag 1021 cctttctaga tgatttaagc cagaagttaa aacccttggg tgaagcagaa cgagagttta 1081 ttttgaattt gaagaaaaag gaatgcaaag acaggggttt tgaatatgat gggaaaatca 1141 atgcctggga tctatattac tacatgactc agacagagga actcaagtat tccatagacc 1201 aagagttcct caaggaatac ttcccaattg aggtggtcac tgaaggcttg ctgaacacct 1261 accaggagtt gttgggactt tcatttgaac aaatgacaga tgctcatgtt tggaacaaga 1321 gtgttacact ttatactgtg aaggataaag ctacaggaga agtattggga cagttctatt 1381 tggacctcta tccaagggaa ggaaaataca atcatgcggc ctgcttcggt ctccagcctg 1441 gctgccttct gcctgatgga agccggatga tggcagtggc tgccctcgtg gtgaacttct 1501 cacagccagt ggcaggtcgt ccctctctcc tgagacacga cgaggtgagg acttactttc 1561 atgagtttGG TCACGTGATG CATCAGATTT GTGCACAGGT GAGTTTTTTT TTTCCCCCAG 1621 TAAACCTGCC AATTAGTTTT TTTAGAAAAC TTCTTGACTG CTGTCAGGTT TCTGCTCCTA 1681 ACAGGTTTTT TCAGAAGCTG AATGTGTTCA AGAATTTTAT AGGCCTTCTG CAAAACCAAA 1741 ATTTTCCTTA TCCTGTCCTC TTTCACAACA TATTTTTACT TGGAGGTTCA TCTACCAGTG 1801 AAAGATGTTT GTTGAATAAC AGCCTATGGA AAAAGAATAA GACTATCTGT AAGGAAAGGT 1861 TACAAATACT TTGCTTTTTC CTTTTAATCC TTTAGAACCC AGCTTTCTTG TACAAAGTGG 1921 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1981 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2041 ACGATCCTAC GTGCCACGCC GATTCACGCG TTAAGTCgac aatcaacctc tggattacaa 2101 aatttgtgaa agatt