Construct: ORF TRCN0000488249
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019151.1_s317c1
- DNA Barcode:
- CTATGTCTGATTGACATCGAGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHRM1 (1128)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488249
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1128 | CHRM1 | cholinergic receptor muscar... | NM_000738.3 | 100% | 100% | |
| 2 | human | 1128 | CHRM1 | cholinergic receptor muscar... | XM_011544742.2 | 100% | 100% | |
| 3 | mouse | 12669 | Chrm1 | cholinergic receptor, musca... | NM_001112697.1 | 91.4% | 98.9% | (many diffs) |
| 4 | mouse | 12669 | Chrm1 | cholinergic receptor, musca... | NM_007698.3 | 91.4% | 98.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1455
- ORF length:
- 1380
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgaac acttcagccc cacctgctgt cagccccaac atcaccgtcc 121 tggcaccagg aaagggtccc tggcaagtgg ccttcattgg gatcaccacg ggcctcctgt 181 cgctagccac agtgacaggc aacctgctgg tactcatctc tttcaaggtc aacacggagc 241 tcaagacagt caataactac ttcctgctga gcctggcctg tgctgacctc atcatcggta 301 ccttctccat gaacctctat accacgtacc tgctcatggg ccactgggct ctgggcacgc 361 tggcttgtga cctctggctg gccctggact atgtggccag caatgcctcc gtcatgaatc 421 tgctgctcat cagctttgac cgctacttct ccgtgactcg gcccctgagc taccgtgcca 481 agcgcacacc ccgccgggca gctctgatga tcggcctggc ctggctggtt tcctttgtgc 541 tctgggcccc agccatcctc ttctggcagt acctggtagg ggagcggaca gtgctagctg 601 ggcagtgcta catccagttc ctctcccagc ccatcatcac ctttggcaca gccatggctg 661 ccttctacct ccctgtcaca gtcatgtgca cgctctactg gcgcatctac cgggagacag 721 agaaccgagc acgggagctg gcagcccttc agggctccga gacgccaggc aaagggggtg 781 gcagcagcag cagctcagag aggtctcagc caggggctga gggctcacca gagactcctc 841 caggccgctg ctgtcgctgc tgccgggccc ccaggctgct gcaggcctac agctggaagg 901 aagaagagga agaggacgaa ggctccatgg agtccctcac atcctcagag ggagaggagc 961 ctggctccga agtggtgatc aagatgccaa tggtggaccc cgaggcacag gcccccacca 1021 agcagccccc acggagctcc ccaaatacag tcaagaggcc gactaagaaa gggcgtgatc 1081 gagctggcaa gggccagaag ccccgtggaa aggagcagct ggccaagcgg aagaccttct 1141 cgctggtcaa ggagaagaag gcggctcgga ccctgagtgc caTCCTCCTG GCCTTCATCC 1201 TCACCTGGAC ACCGTACAAC ATCATGGTGC TGGTGTCCAC CTTCTGCAAG GACTGTGTTC 1261 CCGAGACCCT GTGGGAGCTG GGCTACTGGC TGTGCTACGT CAACAGCACC ATCAACCCCA 1321 TGTGCTACGC ACTCTGCAAC AAAGCCTTCC GGGACACCTT TCGCCTGCTG CTGCTTTGCC 1381 GCTGGGACAA GAGACGCTGG CGCAAGATCC CCAAGCGCCC TGGCTCCGTG CACCGCACTC 1441 CCTCCCGCCA ATGCGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1501 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1561 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTATGTCTGA TTGACATCGA 1621 GGCAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt