Construct: ORF TRCN0000488259
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019432.1_s317c1
- DNA Barcode:
- CTCATGGCCGCTCACCTGGACGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- S1PR3 (1903)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488259
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1903 | S1PR3 | sphingosine-1-phosphate rec... | NM_005226.4 | 100% | 100% | |
| 2 | mouse | 13610 | S1pr3 | sphingosine-1-phosphate rec... | NM_010101.4 | 85.1% | 87.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1209
- ORF length:
- 1134
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggca actgccctcc cgccgcgtct ccagccggtg cgggggaacg 121 agaccctgcg ggagcattac cagtacgtgg ggaagttggc gggcaggctg aaggaggcct 181 ccgagggcag cacgctcacc accgtgctct tcttggtcat ctgcagcttc atcgtcttgg 241 agaacctgat ggttttgatt gccatctgga aaaacaataa atttcacaac cgcatgtact 301 ttttcattgg caacctggct ctctgcgacc tgctggccgg catcgcttac aaggtcaaca 361 ttctgatgtc tggcaagaag acgttcagcc tgtctcccac ggtctggttc ctcagggagg 421 gcagtatgtt cgtggccctt ggggcgtcca cctgcagctt actggccatc gccatcgagc 481 ggcacttgac aatgatcaaa atgaggcctt acgacgccaa caagaggcac cgcgtcttcc 541 tcctgatcgg gatgtgctgg ctcattgcct tcacgctggg cgccctgccc attctgggct 601 ggaactgcct gcacaatctc cctgactgct ctaccatcct gcccctctac tccaagaagt 661 acattgcctt ctgcatcagc atcttcacgg ccatcctggt gaccatcgtg atcctctacg 721 cacgcatcta cttcctggtg aagtccagca gccgtaaggt ggccaaccac aacaactcgg 781 agcggtccat ggcactgctg cggaccgtgg tgattgtggt gagcgtgttc atcgcctgct 841 ggtccccact cttcatcctc ttcctcattg atgtggccTG CAGGGTGCAG GCGTGCCCCA 901 TCCTCTTCAA GGCTCAGTGG TTCATCGTGT TGGCTGTGCT CAACTCCGCC ATGAACCCGG 961 TCATCTACAC GCTGGCCAGC AAGGAGATGC GGCGGGCCTT CTTCCGTCTG GTCTGCAACT 1021 GCCTGGTCAG GGGACGGGGG GCCCGCGCCT CACCCATCCA GCCTGCGCTC GACCCAAGCA 1081 GAAGTAAATC AAGCAGCAGC AACAATAGCA GCCACTCTCC GAAGGTCAAG GAAGACCTGC 1141 CCCACACAGC CCCCTCATCC TGCATCATGG ACAAGAACGC AGCACTTCAG AATGGGATCT 1201 TCTGCAACAA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1261 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1321 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTCATG GCCGCTCACC TGGACGCCAC 1381 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt