Construct: ORF TRCN0000488293
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019940.1_s317c1
- DNA Barcode:
- CTTTGCCTCGCCGCCTGGTGTCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM11 (8834)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488293
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8834 | TMEM11 | transmembrane protein 11 | NM_003876.2 | 99.6% | 100% | 575_576delTA |
| 2 | human | 8834 | TMEM11 | transmembrane protein 11 | NR_024547.1 | 34% | 1_443del;506_797del;1310_1688del | |
| 3 | mouse | 216821 | Tmem11 | transmembrane protein 11 | NM_173453.3 | 89.9% | 96.8% | (many diffs) |
| 4 | mouse | 216821 | Tmem11 | transmembrane protein 11 | NM_001168507.1 | 82.6% | 88% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 648
- ORF length:
- 576
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccgct tggggaagga ggcgtcttgg cccgggcagc agtggcggca 121 gcgcccgaga gagggtgagc ttgtcggcca cagactgcta cattgtgcat gagatctaca 181 atggggagaa tgcccaagac cagtttgagt acgagctgga gcaggccctg gaagcccagt 241 acaagtacat tgtgattgag cccactcgca ttggcgacga gacagcccgc tggatcaccg 301 tgggcaactg cctgcacaag acggccgtgc tggcgggcac cgcctgcctc ttcaccccgt 361 tGGCGCTGCC CTTAGATTAT TCCCACTACA TTTCCCTGCC CGCTGGTGTG CTGAGCCTGG 421 CCTGCTGCAC CCTCTATGGG ATCTCCTGGC AGTTTGACCC TTGCTGCAAG TACCAAGTGG 481 AGTACGACGC CTATAAACTG TCGCGCCTGC CTCTGCACAC ACTCACCTCC TCCACCCCGG 541 TGGTGCTGGT CCGGAAGGAC GACCTGCACA GAAAGAGACT GCACAACACG ATAGCACTGG 601 CCGCCCTGGT GTACTGTGTA AAGAAGATTT ACGAACTCTA TGCCGTAGAC CCAGCTTTCT 661 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 721 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 781 GTGGAAAGGA CGACTTTGCC TCGCCGCCTG GTGTCCAACG CGTTAAGTCg acaatcaacc 841 tctggattac aaaatttgtg aaagatt