Construct: ORF TRCN0000488314
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021917.1_s317c1
- DNA Barcode:
- GATATATCCCTACATTACTATCAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RCSD1 (92241)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488314
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92241 | RCSD1 | RCSD domain containing 1 | NM_052862.4 | 100% | 100% | |
| 2 | human | 92241 | RCSD1 | RCSD domain containing 1 | NM_001322923.1 | 92.7% | 92.7% | 103_104ins90 |
| 3 | human | 92241 | RCSD1 | RCSD domain containing 1 | NM_001322924.2 | 87% | 87% | 106_107ins162 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1320
- ORF length:
- 1248
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaggaa agaccggcag agaccaatgc caatgtggac aactcggcgt 121 ccccctcggt ggcccagctg gccgggcggt ttagggagca ggcggctgca gccaaggaga 181 caccagccag taaaccaacc cgaaggaaac cgccctgttc cctccccctg ttccccccca 241 aggtagacct gggccagaat ggtgaggaga aatcaccacc caatgcgagc caccctccta 301 aattcaaggt caagagctcg cctctgattg agaagcttca ggccaattta acctttgacc 361 cagctgctct actgcctggg gcctcaccca agagtcctgg actcaaggct atggtgtcgc 421 catttcacag cccaccttct acccccagca gccctggtgt gcgatctagg cccagcgagg 481 cagaggaggt gcctgtcagc ttcgaccagc cccctgaagg cagtcatctg ccctgttaca 541 acaaggtgcg gacgaggggc tcaataaaaa ggcgccctcc ctccaggcga ttccgaaggt 601 cacagtcaga ctgtggagaa cttggagatt tcagggcggt ggagtcatct cagcagaacg 661 gtgctaagga agaggatggg gatgaagtgt tgccatccaa gagcaaggcc ccaggatccc 721 ctttgtccag tgagggagca gcgggagagg gagtgagaac cctgggacct gctgaaaagc 781 ctcctctgag gaggtcaccc agcaggacag agaagcagga ggaggacagg gccacagagg 841 aagccaagaa cggtgaaaag gccaggcgga gttcagagga ggtggacggc cagcacccgg 901 cccaagagga ggtcccggaa tcgccccaga cctctggccc agaggcagaa aataggtgtg 961 ggagccccag ggaggaaaag ccagctggag aggaagcaga gatggaaaag gctacagagg 1021 tgaagGGGGA GAGGGTGCAA AATGAAGAGG TGGGACCTGA ACATGACAGC CAAGAAACAA 1081 AGAAGCTGGA GGAGGGAGCT GCAGTGAAGG AGACCCCCCA CAGTCCCCCT GGAGGAGTGA 1141 AGGGCGGAGA TGTCCCCAAG CAGGAAAAAG GCAAGGAAAA ACAACAGGAG GGGGCAGTGC 1201 TCGAGCCAGG CTGCAGCCCC CAGACCGGCC CTGCCCAGCT GGAGACCAGC AGTGAGGTCC 1261 AGAGCGAGCC AGCAGTCCCC AAGCCGGAGG ATGACACTCC TGTCCAGGAC ACTAAAATGT 1321 GAGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1381 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1441 GGCTTTATAT ATCTTGTGGA AAGGACGAGA TATATCCCTA CATTACTATC AGACGCGTTA 1501 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt