Construct: ORF TRCN0000488320
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021124.1_s317c1
- DNA Barcode:
- AACCTCCATCCCGAATTCTCGCCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ROM1 (6094)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488320
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6094 | ROM1 | retinal outer segment membr... | NM_000327.3 | 99.8% | 100% | 669G>C;676C>T |
| 2 | mouse | 19881 | Rom1 | rod outer segment membrane ... | NM_009073.4 | 85.1% | 84.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1125
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcgccg gtgttgcccc tggtgctgcc cctgcagccc cgcatccgcc 121 tggcacaagg gctctggctc ctctcctggc tgctggcgct ggctggtggc gtcatcctcc 181 tctgtagtgg gcacctcctg gtccagctaa ggcaccttgg caccttcctg gctccctcct 241 gtcagttccc tgtcctgccc caggctgccc tggcagcggg cgcggtggct ctgggcacag 301 gactagtggg tgtaggagcc agccgggcaa gtctgaatgc agctctatac cctccctggc 361 gaggggtcct gggcccgctg ctggtggctg gcacggctgg tggggggggg ctcctggtcg 421 tcgccctcgg gctagccctg gctttgcctg ggagtctgga tgaggcgctg gaggagggcc 481 tggtgactgc cttggctcac tacaaggaca cagaggtgcc tgggcactgt caggccaaaa 541 ggctggtgga tgagctgcaa ctgaggtacc actgctgcgg gcgccacggg tacaaggatt 601 ggtttggggt ccagtgggtc agcagccgtt acctggatcc cggtgaccgg gatgtggctg 661 accggatcca gagcaatgta gaaggcctat acctgactga tggggtccct ttctcctgtt 721 gcaaccccca ctcaccccgc ccttgcttgc aaaaccgtct ttcagactcc tacgcccacc 781 ccctgttcga tccccgacaa cccaaccaaa accTCTGGGC CCAAGGGTGC CATGAGGTGC 841 TGCTGGAGCA CTTGCAGGAC TTGGCAGGCA CACTGGGTAG CATGCTGGCT GTCACCTTCC 901 TACTGCAGGC TCTGGTGCTC CTTGGCCTGC GGTACCTGCA AACAGCACTG GAGGGGCTTG 961 GAGGGGTCAT TGATGCGGGA GGAGAGACCC AGGGCTATCT CTTTCCCAGT GGGCTGAAAG 1021 ATATGCTGAA AACAGCATGG CTACAGGGAG GGGTTGCCTG CAGGCCAGCA CCTGAGGAGG 1081 CCCCACCAGG AGAAGCACCT CCCAAGGAGG ATCTATCTGA GGCCTAGAAC CCAGCTTTCT 1141 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1201 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1261 GTGGAAAGGA CGAAACCTCC ATCCCGAATT CTCGCCTACG CGTTAAGTCg acaatcaacc 1321 tctggattac aaaatttgtg aaagatt