Construct: ORF TRCN0000488328
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020571.1_s317c1
- DNA Barcode:
- TTCGGCCGTGGACCCAATGGCCAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- IRAK3 (11213)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488328
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11213 | IRAK3 | interleukin 1 receptor asso... | NM_007199.3 | 99.9% | 99.8% | 439A>G |
| 2 | human | 11213 | IRAK3 | interleukin 1 receptor asso... | NM_001142523.2 | 89.7% | 89.5% | 132_133ins183;256A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1860
- ORF length:
- 1788
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcgggg aactgtgggg cccgcggcgc gctgtcggcg cacacgctgc 121 tgttcgacct gccgcccgcg ctgctcggag agctctgcgc tgttctggac agctgcgacg 181 gcgcgctggg ctggcgcggc ctggcagaga gactttcaag cagctggctg gatgttcgtc 241 atattgaaaa gtatgtagac caaggtaaaa gtggaacaag agaattactt tggtcctggg 301 cacagaaaaa caagaccatc ggtgaccttt tacaggtcct ccaggagatg ggacatcgtc 361 gagctattca tttaattaca aactatggag cagtgttgag tccttcagag aagagttatc 421 aggaaggtgg atttccaaat atattattca aggaaacagc caatgtcacc gtggataatg 481 ttcttattcc tgaacataat gaaaaaggag tactgcttaa atcttccatc agctttcaaa 541 atatcataga aggaactaga aatttccaca aagacttcct aattggagaa ggagagattt 601 ttgaggtata cagagtggag attcaaaacc taacatatgc tgtcaaatta tttaaacagg 661 agaaaaaaat gcagtgtaag aagcattgga agaggttttt atctgagctt gaagttttac 721 tactgtttca tcacccaaac atactagagt tggctgcata ttttacagag actgagaagt 781 tctgtctgat ttatccatac atgagaaatg gaacactttt tgacagattg cagtgtgtag 841 gtgacacggc cccactccct tggcacattc gaatcggtat attaatagga atatccaaag 901 ccattcacta cctgcacaac gttcaaccat gctcggtcat ctgtggcagt atatcaagtg 961 caaacatcct tttggatgat cagtttcaac ccaaactaac tgattttgcc atggcacact 1021 tccggtccca cctagaacat cagagttgta ccataaatat gaccagcagc agcagtaaac 1081 atctgtggta catgccagaa gagtacatca gacaggggaa actttccatt aaaacagatg 1141 tctacagctt tggaattgta ataatggaag ttctaacagg atgtagagta gtgttagatg 1201 atccaaaaca tatccagctg cgggatctcc ttagagaatt gatggagaag agaggcctgg 1261 attcatgtct ctcatttcta gataagaaag tgcctccctg ccctcggaat ttctctgcca 1321 agctcttctg tttggcaggc cggtgtgctg caacgcgggc aaagttaaga ccatcaatgg 1381 atgaagtttt aaatactctt gaaagtactc aagccagctt gtattttgct gaagatcctc 1441 ccacatcact aaagtccttc aggtgtcctt ctcctctatt cctggagaat gtaccaagta 1501 ttccagtgga agatgatgaa agccagaata acaatttact aCCTTCTGAT GAAGGCCTGA 1561 GGATAGACAG AATGACTCAG AAAACTCCTT TTGAATGCAG CCAGTCTGAG GTTATGTTTC 1621 TGAGCTTGGA CAAAAAGCCA GAGAGCAAGA GAAATGAGGA AGCTTGCAAC ATGCCCAGTT 1681 CTTCTTGTGA AGAAAGTTGG TTCCCAAAGT ATATAGTTCC ATCCCAGGAC TTAAGGCCCT 1741 ATAAGGTAAA TATAGATCCT TCTTCAGAAG CTCCAGGGCA TTCTTGCAGG AGCAGGCCAG 1801 TGGAGAGCAG CTGTTCCTCC AAATTTTCCT GGGATGAATA TGAACAGTAC AAAAAAGAAT 1861 AGAACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1921 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1981 GGCTTTATAT ATCTTGTGGA AAGGACGATT CGGCCGTGGA CCCAATGGCC AGACGCGTTA 2041 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt