Construct: ORF TRCN0000488364
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019688.1_s317c1
- DNA Barcode:
- AAGTTTTACCAGCTTAGATAGGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLK3 (1198)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488364
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1198 | CLK3 | CDC like kinase 3 | NM_003992.4 | 99.9% | 99.7% | 1470_1471insG |
| 2 | human | 1198 | CLK3 | CDC like kinase 3 | NM_001130028.1 | 76.7% | 76.6% | 1_444del;1914_1915insG |
| 3 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_006510739.3 | 92.6% | 98.5% | (many diffs) |
| 4 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_017313060.1 | 92.6% | 98.5% | (many diffs) |
| 5 | mouse | 102414 | Clk3 | CDC-like kinase 3 | NM_007713.4 | 71.1% | 75.7% | (many diffs) |
| 6 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_017313061.1 | 51.6% | 54.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1548
- ORF length:
- 1473
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgcat cactgtaagc gataccgctc ccctgaacca gacccgtacc 121 tgagctaccg atggaagagg aggaggtcct acagtcggga acatgaaggg agactgcgat 181 acccgtcccg aagggagcct cccccacgaa gatctcggtc cagaagccat gaccgcctgc 241 cctaccagag gaggtaccgg gagcgccgtg acagcgatac ataccggtgt gaagagcgga 301 gcccatcctt tggagaggac tactatggac cttcacgttc tcgtcatcgt cggcgatcgc 361 gggagagggg gccataccgg acccgcaagc atgcccacca ctgccacaaa cgccgcacca 421 ggtcttgtag cagcgcctcc tcgagaagcc aacagagcag taagcgcagc agccggagtg 481 tggaagatga caaggagggt cacctggtgt gccggatcgg cgattggctc caagagcgat 541 atgagattgt ggggaacctg ggtgaaggca cctttggcaa ggtggtggag tgcttggacc 601 atgccagagg gaagtctcag gttgccctga agatcatccg caacgtgggc aagtaccggg 661 aggctgcccg gctagaaatc aacgtgctca aaaaaatcaa ggagaaggac aaagaaaaca 721 agttcctgtg tgtcttgatg tctgactggt tcaacttcca cggtcacatg tgcatcgcct 781 ttgagctcct gggcaagaac acctttgagt tcctgaagga gaataacttc cagccttacc 841 ccctaccaca tgtccggcac atggcctacc agctctgcca cgcccttaga tttctgcatg 901 agaatcagct gacccataca gacttgaaac cagagaacat cctgtttgtg aattctgagt 961 ttgaaaccct ctacaatgag cacaagagct gtgaggagaa gtcagtgaag aacaccagca 1021 tccgagtggc tgactttggc agtgccacat ttgaccatga gcaccacacc accattgtgg 1081 ccacccgtca ctatcgcccg cctgaggtga tccttgagct gggctgggca cagccctgtg 1141 acgtctggag cattggctgc attctctttg agtactaccg gggcttcaca ctcttccaga 1201 cccacgaaaa ccgagagcac ctggtgatga tggagaagaT CCTAGGGCCC ATCCCATCAC 1261 ACATGATCCA CCGTACCAGG AAGCAGAAAT ATTTCTACAA AGGGGGCCTA GTTTGGGATG 1321 AGAACAGCTC TGACGGCCGG TATGTGAAGG AGAACTGCAA ACCTCTGAAG AGTTACATGC 1381 TCCAAGACTC CCTGGAGCAC GTGCAGCTGT TTGACCTGAT GAGGAGGATG TTAGAATTTG 1441 ACCCTGCCCA GCGCATCACA CTGGCCGAGG CCCTGCTGCA CCCCTTCTTT GCTGGCCTGA 1501 CCCCTGAGGA GCGGTCCTTC CACACCAGCC GCAACCCAAG CAGAGACCCA GCTTTCTTGT 1561 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1621 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1681 GAAAGGACGA AAGTTTTACC AGCTTAGATA GGATACGCGT TAAGTCgaca atcaacctct 1741 ggattacaaa atttgtgaaa gatt