Construct: ORF TRCN0000488379

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020177.1_s317c1
DNA Barcode:
CTCGATCTCGCGAGCGCCATGGCC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
PMVK (10654)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488379
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10654 PMVK phosphomevalonate kinase NM_006556.4 100% 100%
2 human 10654 PMVK phosphomevalonate kinase NM_001323011.3 84.4% 86.9% (many diffs)
3 human 10654 PMVK phosphomevalonate kinase NM_001323012.3 60.9% 60.9% 0_1ins225
4 human 10654 PMVK phosphomevalonate kinase NM_001348696.2 60.9% 53.6% 0_1ins106;50_51ins119
5 mouse 68603 Pmvk phosphomevalonate kinase NM_026784.3 86.6% 83.3% (many diffs)
6 mouse 68603 Pmvk phosphomevalonate kinase NM_001310640.1 52.4% 52% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
648
ORF length:
576
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggccccg ctgggaggcg ccccgcggct ggtactgctg ttcagcggca
121agaggaaatc cgggaaggac ttcgtgaccg aggcgctgca gagcagactt ggagctgatg
181tctgtgctgt cctccggctc tctggtccac tcaaggaaca gtatgctcag gagcatggct
241tgaacttcca gagactcctg gacaccagca cctacaagga ggcctttcgg aaggacatga
301tccgctgggg agaggagaaa cgccaggctg acccaggctt ctttTGCAGG AAGATTGTGG
361AGGGCATCTC CCAGCCCATC TGGCTGGTGA GTGACACACG GAGAGTGTCT GACATCCAGT
421GGTTTCGGGA GGCCTATGGG GCCGTGACGC AGACGGTCCG CGTTGTAGCG TTGGAGCAGA
481GCCGACAGCA GCGGGGCTGG GTGTTCACGC CAGGGGTGGA CGATGCTGAG TCAGAATGTG
541GCCTGGACAA CTTCGGGGAC TTTGACTGGG TCATCGAGAA CCATGGAGTT GAACAGCGCC
601TGGAGGAGCA GTTGGAGAAC CTGATAGAAT TTATCCGCTC CAGACTTTGA GACCCAGCTT
661TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
721CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
781CTTGTGGAAA GGACGACTCG ATCTCGCGAG CGCCATGGCC ACGCGTTAAG TCgacaatca
841acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.