Construct: ORF TRCN0000488381
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019213.1_s317c1
- DNA Barcode:
- ATTAATAGTATCCAAGTATCCGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAS2R20 (259295)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488381
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 259295 | TAS2R20 | taste 2 receptor member 20 | NM_176889.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1002
- ORF length:
- 927
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgatg agttttctac acattgtttt ttccattcta gtagtggttg 121 catttattct tggaaatttt gccaatggct ttatagcact gataaatttc attgcctggg 181 tcaagagaca aaagatctcc tcagctgatc aaattattgc tgctctggca gtctccagag 241 ttggtttgct ctgggtaata ttattacatt ggtattcaac tgtgttgaat ccaacttcat 301 ctaatttaaa agtaataatt tttatttcta atgcctgggc agtaaccaat catttcagca 361 tctggcttgc tactagcctc agcatatttt atttgctcaa gatcgtcaat ttctccagac 421 ttatttttca tcacttaaaa aggaaggcta agagtgtagt tctggtgata gtgttggggt 481 ctttgttctt tttggtttgt caccttgtga tgaaacacac gtatataaat gtgtggacag 541 aagaatgtga aggaaacgta acttggaaga tcaaactgag gaatgcaatg cacctttcca 601 acttgactgt agccatgcta gcaaacttga taccattcac tctgaccctg atatcttttc 661 tgctgttaat ctactctctg tgtaaacatc tgaagaagat gcagctccat ggcaaaggat 721 ctcaagatcc cagcaccaag atccacataa aagctctgca aactgtgacc TCCTTCCTCA 781 TATTACTTGC CATTTACTTT CTGTGTCTAA TCATATCGTT TTGGAATTTT AAGATGCGAC 841 CAAAAGAAAT TGTCTTAATG CTTTGCCAAG CTTTTGGAAT CATATATCCA TCATTCCACT 901 CATTCATTCT GATTTGGGGG AACAAGACGC TAAAGCAGAC CTTTCTTTCA GTTTTGTGGC 961 AGGTGACTTG CTGGGCAAAA GGACAGAACC AGTCAACTCC AGACCCAGCT TTCTTGTACA 1021 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1081 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1141 AGGACGAATT AATAGTATCC AAGTATCCGA CACGCGTTAA GTCgacaatc aacctctgga 1201 ttacaaaatt tgtgaaagat t