Construct: ORF TRCN0000488395
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021622.1_s317c1
- DNA Barcode:
- TGTTTACGCGCCCATCTTCCCTAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- EIF2S1 (1965)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488395
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1965 | EIF2S1 | eukaryotic translation init... | NM_004094.5 | 100% | 100% | |
| 2 | mouse | 13665 | Eif2s1 | eukaryotic translation init... | NM_026114.3 | 93.3% | 99% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1014
- ORF length:
- 945
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gccgggtcta agttgtagat tttatcaaca caaatttcct gaggtggaag 121 atgtagtgat ggtgaatgtc agatccattg ctgaaatggg ggcttatgtc agcttgctgg 181 aatacaacaa cattgaaggc atgattcttc ttagtgaatt atccagaagg cgtatccgtt 241 ctatcaacaa actcatccga attggcagga atgagtgtgt ggttgtcatt agggtggaca 301 aagaaaaagg atatattgat ttgtcaaaaa gaagagtttc tccagaggaa gcaatcaaat 361 gtgaagacaa attcacaaaa tccaaaactg tttatagcat tcttcgtcat gttgctgagg 421 tgttagaata caccaaggat gagcagctgg aaagcctatt ccagaggact gcctgggtct 481 ttgatgacaa gtacaagaga cctggatatg gtgcctatga tgcatttaag catgcagtct 541 cagacccatc tattttggat agtttagatt tgaatgaaga tgaacgggaa gtactcatta 601 ataatattaa taggcgcttg accccacagg ctgtcaaaat tcgagcagat attgaagtgg 661 cttgttatgg ttatgaaggc attgatgctg taaaagaagc ccTAAGAGCA GGTTTGAATT 721 GTTCTACAGA AAACATGCCC ATTAAGATTA ATCTAATAGC TCCTCCTCGG TATGTAATGA 781 CTACGACAAC CCTGGAGAGA ACAGAAGGCC TTTCTGTCCT CAGTCAAGCT ATGGCTGTTA 841 TCAAAGAGAA GATTGAGGAA AAGAGGGGTG TGTTCAATGT TCAAATGGAG CCCAAAGTGG 901 TCACAGATAC AGATGAGACT GAACTTGCGA GGCAGATGGA GAGGCTTGAA AGAGAAAATG 961 CCGAAGTGGA TGGAGATGAT GATGCAGAAG AAATGGAAGC CAAAGCTGAA GATTAGAACC 1021 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1081 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1141 ATATATCTTG TGGAAAGGAC GATGTTTACG CGCCCATCTT CCCTAGACGC GTTAAGTCga 1201 caatcaacct ctggattaca aaatttgtga aagatt