Construct: ORF TRCN0000488415
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019269.1_s317c1
- DNA Barcode:
- CCTTCGGACTAAATACTAGACATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOMER1 (9456)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488415
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9456 | HOMER1 | homer scaffold protein 1 | NM_004272.5 | 100% | 100% | |
| 2 | human | 9456 | HOMER1 | homer scaffold protein 1 | NM_001277077.1 | 63.2% | 63.2% | 294_295ins390 |
| 3 | human | 9456 | HOMER1 | homer scaffold protein 1 | XM_017010059.2 | 50.7% | 49.5% | (many diffs) |
| 4 | human | 9456 | HOMER1 | homer scaffold protein 1 | NM_001277078.1 | 49.8% | 49.4% | 528_529delGAinsTTC;531_532ins530 |
| 5 | mouse | 26556 | Homer1 | homer scaffolding protein 1 | NM_001284189.2 | 91.1% | 98.5% | (many diffs) |
| 6 | mouse | 26556 | Homer1 | homer scaffolding protein 1 | NM_147176.4 | 88.1% | 95.3% | (many diffs) |
| 7 | mouse | 26556 | Homer1 | homer scaffolding protein 1 | NM_152134.3 | 87% | 93.7% | (many diffs) |
| 8 | mouse | 26556 | Homer1 | homer scaffolding protein 1 | NM_001347598.1 | 50.3% | 50.4% | (many diffs) |
| 9 | mouse | 26556 | Homer1 | homer scaffolding protein 1 | NM_011982.4 | 47.8% | 49.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1137
- ORF length:
- 1062
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgggg gaacaaccta tcttcagcac tcgagctcat gtcttccaaa 121 ttgacccaaa cacaaagaag aactgggtac ccaccagcaa gcatgcagtt actgtgtctt 181 atttctatga cagcacaaga aatgtgtata ggataatcag tttagatggc tcaaaggcaa 241 taataaatag taccatcacc ccaaacatga catttactaa aacatctcag aagtttggcc 301 agtgggctga tagccgggca aacaccgttt atggattggg attctcctct gagcatcatc 361 tttcgaaatt tgcagaaaag tttcaggaat ttaaagaagc tgctcgacta gcaaaggaaa 421 aatcacaaga gaagatggaa cttaccagta caccttcaca ggaatccgca ggcggggatc 481 ttcagtctcc tttaacaccg gaaagtatca acgggacaga tgatgaaaga acacctgatg 541 tgacacagaa ctcagagcca agggctgaac caactcagaa tgcattgcca ttttcacata 601 gttcagcaat cagcaaacat tgggaggctg aactggctac cctcaaagga aataatgcca 661 aactcactgc agccctgctg gagtccactg ccaatgtgaa acaatggaaa cagcaacttg 721 ctgcctatca agaggaagca gaacgtctgc acaagcgggt gactgaactt gaatgtgtta 781 gtagccaagc aaatgcagta catactcata agacagaatt aaatcagaCA ATACAAGAAC 841 TGGAAGAGAC ACTGAAACTG AAGGAAGAGG AAATAGAAAG GTTAAAACAA GAAATTGATA 901 ATGCCAGAGA ACTACAAGAA CAGAGGGATT CTTTGACTCA GAAACTACAG GAAGTAGAAA 961 TTCGGAACAA AGACCTGGAG GGACAACTGT CTGACTTAGA GCAACGTCTG GAGAAAAGTC 1021 AGAATGAACA AGAAGCTTTT CGCAATAACC TGAAGACACT CTTAGAAATT CTGGATGGAA 1081 AGATATTTGA ACTAACAGAA TTACGAGATA ACTTGGCCAA GCTACTAGAA TGCAGCGACC 1141 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1201 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1261 ATATATCTTG TGGAAAGGAC GACCTTCGGA CTAAATACTA GACATGACGC GTTAAGTCga 1321 caatcaacct ctggattaca aaatttgtga aagatt