Construct: ORF TRCN0000488423
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019604.1_s317c1
- DNA Barcode:
- GTGCAGAGAACCGCACACCTAGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TACR2 (6865)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488423
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6865 | TACR2 | tachykinin receptor 2 | NM_001057.3 | 99.8% | 99.4% | 734T>A;1194_1195insG |
| 2 | mouse | 21337 | Tacr2 | tachykinin receptor 2 | NM_009314.4 | 82.1% | 82% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1272
- ORF length:
- 1197
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgggg acctgtgaca ttgtgactga agccaatatc tcatctggcc 121 ctgagagcaa caccacgggc atcacagcct tctccatgcc cagctggcaa ctggcactgt 181 gggccacagc ctacctggcc ctggtgctgg tggccgtgac gggtaatgcc atcgtcatct 241 ggatcatcct ggcccatcgg aggatgcgca cagtcaccaa ctacttcatc gtcaatctgg 301 cgctggctga cctctgcatg gctgccttca atgccgcctt caactttgtc tatgccagcc 361 acaacatctg gtactttggc cgtgccttct gctacttcca gaacctcttc cccatcacag 421 ccatgtttgt cagcatctac tccatgaccg ccattgctgc cgacaggtac atggccatcg 481 tccacccctt ccagcctcgg ctttcagctc ccagcaccaa ggcggttatt gctggcatct 541 ggctggtggc tctcgccctg gcctcccctc agtgcttcta ctccaccgtc accatggacc 601 agggtgccac caagtgcgtg gtggcctggc ccgaagacag cgggggcaag acgctcctcc 661 tgtaccacct cgtggtgatc gccctcatct acttcctgcc gctcgcggtg atgtttgtag 721 cctacagcgt catcggcctc acgctctgga ggcgcgcagt gcccggacat caggcgcacg 781 gtgccaacct gcgccacctg caggccaaga agaagtttgt gaagaccatg gtgctggtgg 841 tgctgacgtt tgccatctgc tggctgccct accacctcta cttcatccTG GGCAGCTTCC 901 AGGAGGACAT CTACTGCCAC AAGTTCATCC AGCAAGTCTA CCTGGCACTC TTCTGGTTGG 961 CCATGAGCTC TACCATGTAC AATCCCATCA TCTACTGCTG TCTCAACCAC AGGTTTCGCT 1021 CTGGATTCCG GCTTGCCTTC CGCTGCTGCC CATGGGTCAC ACCCACCAAG GAAGATAAGC 1081 TCGAGCTGAC TCCCACGACC TCCCTCTCCA CGAGAGTCAA CAGGTGTCAC ACTAAGGAGA 1141 CTTTGTTCAT GGCTGGGGAC ACAGCCCCCT CCGAGGCTAC CAGTGGGGAG GCGGGGCGTC 1201 CCCAGGATGG ATCAGGGCTA TGGTTTGGGT ATGGTTTGCT TGCCCCCACC AAAACTCATG 1261 TTGAAATTGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1321 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1381 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGTGCAG AGAACCGCAC ACCTAGCAAC 1441 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt