Construct: ORF TRCN0000488455
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020910.1_s317c1
- DNA Barcode:
- ATGGGCCGGACGTATCGATTTTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ACKR2 (1238)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488455
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1238 | ACKR2 | atypical chemokine receptor 2 | NM_001296.5 | 99.9% | 99.7% | 1118A>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1224
- ORF length:
- 1152
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccgcc actgcctctc cgcagccact cgccactgag gatgccgatt 121 ctgagaatag cagcttctat tactatgact acctggatga agtggccttc atgctctgca 181 ggaaggatgc agtggtgtcc tttggcaaag tcttcctccc agtcttctat agcctgattt 241 ttgtgttggg cctcagcggg aacctccttc ttctcatggt cttgctccgt tacgtgcctc 301 gcaggcggat ggttgagatc tatctgctga atctggccat ctccaacctt ctgtttctgg 361 tgacactgcc cttctggggc atctccgtgg cctggcattg ggtcttcggg agtttcttgt 421 gcaagatggt gagcactctt tatactatta acttttacag tggcatcttt ttcattagct 481 gcatgagcct ggacaagtac ctggagatcg ttcatgctca gccctaccac aggctgagga 541 cccgggccaa gagcctgctc cttgctacca tagtatgggc tgtgtccctg gccgtctcca 601 tccctgatat ggtctttgta cagacacatg aaaatcccaa gggtgtgtgg aactgccacg 661 cagatttcgg cgggcatggg accatttgga agctcttcct ccgcttccag cagaacctcc 721 tagggtttct ccttccactc cttgccatga tcttcttcta ctcccgtatt ggttgtgtct 781 tggtgaggct gaggcccgca ggccagggcc gggctttaaa aatagctgca gccttggtgg 841 tggccttctt cgtgctatgg ttcccataca atctcacctt gtttctgcat acgctgttGG 901 ACCTGCAAGT ATTCGGGAAC TGTGAGGTCA GCCAGCATCT AGACTACGCA CTCCAGGTAA 961 CAGAGAGCAT CGCCTTCCTT CACTGCTGCT TTTCCCCCAT CCTGTATGCC TTCTCCAGTC 1021 ACCGCTTCCG CCAGTACCTG AAGGCTTTCC TGGCTGCCGT GCTTGGATGG CACCTGGCAC 1081 CTGGCACTGC CCAGGCCTCA TTATCCAGCT GTTCTGAGAG CAGCATACTT ACTGCCCAAG 1141 AGGAAATGAC TGGCATGAAT GACCTTGGAG AGAGGCAGTC TGAGAACTCC CCTAACAAGG 1201 AGGATGTGGG GAATAAATCA GCCTGAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1261 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1321 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATGGGCCG 1381 GACGTATCGA TTTTTAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1441 aagatt