Construct: ORF TRCN0000488477
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021136.1_s317c1
- DNA Barcode:
- GGCCTCGAGATTCCGGTCTGCCGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TAS2R1 (50834)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488477
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50834 | TAS2R1 | taste 2 receptor member 1 | NM_019599.2 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 969
- ORF length:
- 897
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgctagag tctcacctca ttatctattt tcttcttgca gtgatacaat 121 ttcttcttgg gattttcaca aatggcatca ttgtggtggt gaatggcatt gacttgatca 181 agcacagaaa aatggctccg ctggatctcc ttctttcttg tctggcagtt tctagaattt 241 ttctgcagtt gttcatcttc tacgttaatg tgattgttat cttcttcata gaattcatca 301 tgtgttctgc gaattgtgca attctcttat ttataaatga attggaactt tggcttgcca 361 catggctcgg cgttttctat tgtgccaagg ttgccagcgt ccgtcaccca ctcttcatct 421 ggttgaagat gaggatatcc aagctggtcc catggatgat cctggggtct ctgctatatg 481 tatctatgat ttgtgttttc catagcaaat atgcagggtt tatggtccca tacttcctaa 541 ggaaattttt ctcccaaaat gccacaattc aaaaagaaga tacactGGCT ATACAGATTT 601 TCTCTTTTGT TGCTGAGTTC TCAGTGCCAT TGCTTATCTT CCTTTTTGCT GTTTTGCTCT 661 TGATTTTCTC TCTGGGGAGG CACACCCGGC AAATGAGAAA CACAGTGGCC GGCAGCAGGG 721 TTCCTGGCAG GGGTGCACCC ATCAGCGCGT TGCTGTCTAT CCTGTCCTTC CTGATCCTCT 781 ACTTCTCCCA CTGCATGATA AAAGTTTTTC TCTCTTCTCT AAAGTTTCAC ATCAGAAGGT 841 TCATCTTTCT GTTCTTCATC CTTGTGATTG GTATATACCC TTCTGGACAC TCTCTCATCT 901 TAATTTTAGG AAATCCTAAA TTGAAACAAA ATGCAAAAAA GTTCCTCCTC CACAGTAAGT 961 GCTGTCAGTA GAACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1021 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1081 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGGC CTCGAGATTC CGGTCTGCCG 1141 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t