Construct: ORF TRCN0000488480
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020855.1_s317c1
- DNA Barcode:
- CGACCTTCGCGACACTTGTTCTTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- S1PR3 (1903)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488480
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1903 | S1PR3 | sphingosine-1-phosphate rec... | NM_005226.4 | 100% | 100% | |
| 2 | mouse | 13610 | S1pr3 | sphingosine-1-phosphate rec... | NM_010101.4 | 85.1% | 87.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1206
- ORF length:
- 1134
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcaact gccctcccgc cgcgtctcca gccggtgcgg gggaacgaga 121 ccctgcggga gcattaccag tacgtgggga agttggcggg caggctgaag gaggcctccg 181 agggcagcac gctcaccacc gtgctcttct tggtcatctg cagcttcatc gtcttggaga 241 acctgatggt tttgattgcc atctggaaaa acaataaatt tcacaaccgc atgtactttt 301 tcattggcaa cctggctctc tgcgacctgc tggccggcat cgcttacaag gtcaacattc 361 tgatgtctgg caagaagacg ttcagcctgt ctcccacggt ctggttcctc agggagggca 421 gtatgttcgt ggcccttggg gcgtccacct gcagcttact ggccatcgcc atcgagcggc 481 acttgacaat gatcaaaatg aggccttacg acgccaacaa gaggcaccgc gtcttcctcc 541 tgatcgggat gtgctggctc attgccttca cgctgggcgc cctgcccatt ctgggctgga 601 actgcctgca caatctccct gactgctcta ccatcctgcc cctctactcc aagaagtaca 661 ttgccttctg catcagcatc ttcacggcca tcctggtgac catcgtgatc ctctacgcac 721 gcatctactt cctggtgaag tccagcagcc gtaaggtggc caaccacaac aactcggagc 781 ggtccatggc actgctgcgg accgtggtga ttgtggtgag cgtgttcatc gcctgctggt 841 ccccactctt catcctcttc ctcattgatg tggcctgcag ggtgcaggcg tgccccatcc 901 TCTTCAAGGC TCAGTGGTTC ATCGTGTTGG CTGTGCTCAA CTCCGCCATG AACCCGGTCA 961 TCTACACGCT GGCCAGCAAG GAGATGCGGC GGGCCTTCTT CCGTCTGGTC TGCAACTGCC 1021 TGGTCAGGGG ACGGGGGGCC CGCGCCTCAC CCATCCAGCC TGCGCTCGAC CCAAGCAGAA 1081 GTAAATCAAG CAGCAGCAAC AATAGCAGCC ACTCTCCGAA GGTCAAGGAA GACCTGCCCC 1141 ACACAGCCCC CTCATCCTGC ATCATGGACA AGAACGCAGC ACTTCAGAAT GGGATCTTCT 1201 GCAACTAGAA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1261 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1321 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGACCT TCGCGACACT TGTTCTTTAC 1381 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt