Construct: ORF TRCN0000488508
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020292.1_s317c1
- DNA Barcode:
- TCAGATACTTTCTTACACCGTTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DCK (1633)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488508
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1633 | DCK | deoxycytidine kinase | NM_000788.3 | 99.4% | 99.2% | 4G>C;780_781insTTG |
| 2 | mouse | 13178 | Dck | deoxycytidine kinase | NM_007832.4 | 87.8% | 92.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 855
- ORF length:
- 783
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcccacc ccgcccaaga gaagctgccc gtctttctca gccagctctg 121 aggggacccg catcaagaaa atctccatcg aagggaacat cgctgcaggg aagtcaacat 181 ttgtgaatat ccttaaacaa ttgtgtgaag attgggaagt ggttcctgaa cctgttgcca 241 gatggtgcaa tgttcaaagt actcaagatg aatttgagga acttacaatg tctcagaaaa 301 atggtgggaa tgttcttcag atgatgtatg agaaacctga acgatggtct tttaccttcc 361 aaacatatgc ctgtctcagt cgaataagag ctcagcttgc ctctctgaat ggcaagctca 421 aagatgcaga gaaacctgta ttattttttg aacgatctgt gtatagtgac aggtatattt 481 ttgcatctaa tttgtatgaa tctgaatgca tgaatgagac agagtggaca atttatcaag 541 actggcatga ctGGATGAAT AACCAATTTG GCCAAAGCCT TGAATTGGAT GGAATCATTT 601 ATCTTCAAGC CACTCCAGAG ACATGCTTAC ATAGAATATA TTTACGGGGA AGAAATGAAG 661 AGCAAGGCAT TCCTCTTGAA TATTTAGAGA AGCTTCATTA TAAACATGAA AGCTGGCTCC 721 TGCATAGGAC ACTGAAAACC AACTTCGATT ATCTTCAAGA GGTGCCTATC TTAACACTGG 781 ATGTTAATGA AGACTTTAAA GACAAATATG AAAGTCTGGT TGAAAAGGTC AAAGAGTTTT 841 TGAGTACTTT GTTGTGAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 901 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 961 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCAGATA CTTTCTTACA 1021 CCGTTTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt