Construct: ORF TRCN0000488520
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021440.1_s317c1
- DNA Barcode:
- CATGGGGTACCAGTGTTGGCCGTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- COMT (1312)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488520
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1312 | COMT | catechol-O-methyltransferase | NM_007310.3 | 100% | 100% | |
| 2 | human | 1312 | COMT | catechol-O-methyltransferase | NM_000754.4 | 81.5% | 81.5% | 1_150del |
| 3 | human | 1312 | COMT | catechol-O-methyltransferase | NM_001135161.2 | 81.5% | 81.5% | 1_150del |
| 4 | human | 1312 | COMT | catechol-O-methyltransferase | NM_001135162.2 | 81.5% | 81.5% | 1_150del |
| 5 | human | 1312 | COMT | catechol-O-methyltransferase | NM_001362828.2 | 81.5% | 81.5% | 1_150del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 735
- ORF length:
- 663
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaag 61 gagatagaac catgggtgac accaaggagc agcgcatcct gaaccacgtg ctgcagcatg 121 cggagcccgg gaacgcacag agcgtgctgg aggccattga cacctactgc gagcagaagg 181 agtgggccat gaacgtgggc gacaagaaag gcaagatcgt ggacgccgtg attcaggagc 241 accagccctc cgtgctgctg gagctggggg cctactgtgg ctactcagct gtgcgcatgg 301 cccgcctgct gtcaccaggg gcgaggctca tcaccatcga gatcaacccc gactgtgccg 361 ccatcaccca gcggatggtg gatttcgctg gcgtgaagga caaggtcacc cttgtggttG 421 GAGCGTCCCA GGACATCATC CCCCAGCTGA AGAAGAAGTA TGATGTGGAC ACACTGGACA 481 TGGTCTTCCT CGACCACTGG AAGGACCGGT ACCTGCCGGA CACGCTTCTC TTGGAGGAAT 541 GTGGCCTGCT GCGGAAGGGG ACAGTGCTAC TGGCTGACAA CGTGATCTGC CCAGGTGCGC 601 CAGACTTCCT AGCACACGTG CGCGGGAGCA GCTGCTTTGA GTGCACACAC TACCAATCGT 661 TCCTGGAATA CAGGGAGGTG GTGGACGGCC TGGAGAAGGC CATCTACAAG GGCCCAGGCA 721 GCGAAGCAGG GCCCTGAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 781 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 841 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACATGGGG TACCAGTGTT 901 GGCCGTTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt