Construct: ORF TRCN0000488525
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021299.1_s317c1
- DNA Barcode:
- TGTACCCCGTTGTATTGACCGAGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TGM4 (7047)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488525
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7047 | TGM4 | transglutaminase 4 | NM_003241.4 | 99.8% | 99.8% | 1057C>T;1086C>T;1524C>T |
| 2 | human | 7047 | TGM4 | transglutaminase 4 | XM_011534042.2 | 93.6% | 93.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2124
- ORF length:
- 2052
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgatggat gcatcaaaag agctgcaagt tctccacatt gacttcttga 121 atcaggacaa cgccgtttct caccacacat gggagttcca aacgagcagt cctgtgttcc 181 ggcgaggaca ggtgtttcac ctgcggctgg tgctgaacca gcccctacaa tcctaccacc 241 aactgaaact ggaattcagc acagggccga atcctagcat cgccaaacac accctggtgg 301 tgctcgaccc gaggacgccc tcagaccact acaactggca ggcaaccctt caaaatgagt 361 ctggcaaaga ggtcacagtg gctgtcacca gttcccccaa tgccatcctg ggcaagtacc 421 aactaaacgt gaaaactgga aaccacatcc ttaagtctga agaaaacatc ctataccttc 481 tcttcaaccc atggtgtaaa gaggacatgg ttttcatgcc tgatgaggac gagcgcaaag 541 agtacatcct caatgacacg ggctgccatt acgtgggggc tgccagaagt atcaaatgca 601 aaccctggaa ctttggtcag tttgagaaaa atgtcctgga ctgctgcatt tccctgctga 661 ctgagagctc cctcaagccc acagatagga gggaccccgt gctggtgtgc agggccatgt 721 gtgctatgat gagctttgag aaaggccagg gcgtgctcat tgggaattgg actggggact 781 acgaaggtgg cacagcccca tacaagtgga caggcagtgc cccgatcctg cagcagtact 841 acaacacgaa gcaggctgtg tgctttggcc agtgctgggt gtttgctggg atcctgacta 901 cagtgctgag agcgttgggc atcccagcac gcagtgtgac aggcttcgat tcagctcacg 961 acacagaaag gaacctcacg gtggacacct atgtgaatga gaatggcgag aaaatcacca 1021 gtatgaccca cgactctgtc tggaatttcc atgtgtggac ggatgcctgg atgaagcgac 1081 cggatctgcc caagggctac gacggctggc aggctgtgga cgcaacgtcg caggagcgaa 1141 gccagggtgt cttctgttgt gggccatcac cactgaccgc catccgcaaa ggtgacatct 1201 ttattgtcta tgacaccaga ttcgtcttct cagaagtgaa tggtgacagg ctcatctggt 1261 tggtgaagat ggtgaatggg caggaggagt tacacgtaat ttcaatggag accacaagca 1321 tcgggaaaaa catcagcacc aaggcagtgg gccaagacag gcggagagat atcacctatg 1381 agtacaagta tccagaaggc tcctctgagg agaggcaggt catggatcat gccttcctcc 1441 ttctcagttc tgagagggag cacagacgac ctgtaaaaga gaactttctt cacatgtcgg 1501 tacaatcaga tgatgtgctg ctgggaaact ctgttaattt caccgtgatt cttaaaagga 1561 agaccgctgc cctacagaat gtcaacatct tgggttcctt tgaactacag ttgtacactg 1621 gcaagaagat ggcaaaactg tgtgacctca ataagacctc gcagatccaa ggtcaagtat 1681 cagaagtgac tctgaccttg gactccaaga cctacatcaa cagcctggct atattagatg 1741 atgagccagt tatcagaggt ttcatcattg cggaaattgt gGAGTCTAAG GAAATCATGG 1801 CCTCTGAAGT ATTCACGTCT TTCCAGTACC CTGAGTTCTC TATAGAGTTG CCTAACACAG 1861 GCAGAATTGG CCAGCTACTT GTCTGCAATT GTATCTTCAA GAATACCCTG GCCATCCCTT 1921 TGACTGACGT CAAGTTCTCT TTGGAAAGCC TGGGCATCTC CTCACTACAG ACCTCTGACC 1981 ATGGGACGGT GCAGCCTGGT GAGACCATCC AATCCCAAAT AAAATGCACC CCAATAAAAA 2041 CTGGACCCAA GAAATTTATC GTCAAGTTAA GTTCCAAACA AGTGAAAGAG ATTAATGCTC 2101 AGAAGATTGT TCTCATCACC AAGTAGAACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 2161 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2221 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATGTACCCC 2281 GTTGTATTGA CCGAGCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2341 aagatt