Construct: ORF TRCN0000488579
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020301.1_s317c1
- DNA Barcode:
- AATTTACAAGTCACCAACTCTCGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- BLK (640)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488579
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 640 | BLK | BLK proto-oncogene, Src fam... | NM_001715.3 | 99.7% | 99.8% | 843T>C;1515_1516insTTG |
| 2 | human | 640 | BLK | BLK proto-oncogene, Src fam... | XM_011543824.1 | 94.8% | 94.9% | 843T>C;952_1029del;1593_1594insTTG |
| 3 | human | 640 | BLK | BLK proto-oncogene, Src fam... | XM_011543825.3 | 94.8% | 94.9% | 843T>C;952_1029del;1593_1594insTTG |
| 4 | human | 640 | BLK | BLK proto-oncogene, Src fam... | NM_001330465.2 | 85.7% | 85.7% | 0_1ins213;630T>C;1302_1303insTTG |
| 5 | human | 640 | BLK | BLK proto-oncogene, Src fam... | XM_011543827.1 | 81.5% | 81.5% | (many diffs) |
| 6 | human | 640 | BLK | BLK proto-oncogene, Src fam... | XM_011543828.3 | 62.6% | 51% | (many diffs) |
| 7 | human | 640 | BLK | BLK proto-oncogene, Src fam... | XM_011543829.3 | 62.4% | 51.1% | (many diffs) |
| 8 | mouse | 12143 | Blk | B lymphoid kinase | NM_007549.2 | 83.7% | 85.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1590
- ORF length:
- 1518
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggggctg gtaagtagca aaaagccgga caaggaaaag ccgatcaaag 121 agaaggacaa gggccaatgg agccccctga aggtcagcgc ccaagacaag gacgccccgc 181 cactgccgcc cctggttgtc ttcaaccacc ttactcctcc accgcccgat gaacacctgg 241 atgaagacaa gcatttcgtg gtggctctgt atgactacac cgctatgaat gatcgggacc 301 tgcagatgct gaagggggag aagctacagg tcctgaaggg aactggagac tggtggctgg 361 ccaggtcact cgtcacagga agagaaggct atgtgcccag taactttgtg gcccgagtgg 421 agagcctgga aatggaaagg tggttcttta gatcacaggg tcggaaggag gctgagaggc 481 agcttcttgc tccaatcaac aaggccggct cctttcttat cagagagagt gaaaccaaca 541 aaggtgcctt ctccctgtct gtgaaggatg tcaccaccca gggggagctg atcaagcact 601 ataagatccg ctgcctggat gaagggggct actacatctc cccccggatc accttcccct 661 cgctccaggc cctggtgcag cactattcta agaaggggga tggtctatgc cagaggctga 721 ccctgccctg tgtgcgcccg gccccgcaga atccctgggc ccaggatgaa tgggagatcc 781 cccggcagtc tctcaggctg gtcaggaaac tcgggtctgg acaattcggc gaagtctgga 841 tgggttacta caaaaacaac atgaaggtgg ccattaagac gctgaaggag ggaaccatgt 901 ctccagaagc cttcctgggt gaggccaacg tgatgaaggc tctgcagcac gagcggctgg 961 tccgactcta cgcagtggtc accaaggagc ccatctacat tgtcaccgag tacatggcca 1021 gaggatgcct gctggatttc ctgaagacag atgaagggag cagattgtca ctcccaaggc 1081 tgattgacat gtcggcgcag attgctgaag ggatggcata cattgagcgc atgaattcca 1141 tccaccgcga cctgcgggcg gccaacatcc tggtgtctga ggccttgtgc tgcaaaattg 1201 ctgattttgg cttggctcga atcatcgaca gtgaatacac ggcccaagag ggggccaagt 1261 tccccatcaa gtggacagcc ccggaagcca tccacttcgg ggtcttcacc atcaaagcag 1321 acgtgtggtc gtttggagtc ctccTGATGG AAGTTGTCAC TTATGGGCGG GTGCCATACC 1381 CAGGGATGAG CAACCCCGAG GTCATCCGCA ACCTGGAGCG CGGCTACCGC ATGCCGCGCC 1441 CCGACACCTG CCCGCCCGAG CTGTACCGCG GCGTCATCGC CGAGTGCTGG CGCAGCCGGC 1501 CCGAGGAGCG GCCCACCTTC GAGTTCCTGC AGTCGGTGCT GGAGGACTTC TACACGGCCA 1561 CCGAGCGGCA GTACGAGCTG CAGCCCTTGT GAGACCCAGC TTTCTTGTAC AAAGTGGTTG 1621 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1681 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 1741 TTTACAAGTC ACCAACTCTC GAACGCGTTA AGTCgacaat caacctctgg attacaaaat 1801 ttgtgaaaga tt