Construct: ORF TRCN0000488640
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020374.1_s317c1
- DNA Barcode:
- CTTTTTCACCTTCCTTGATTGAAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- EPHB3 (2049)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488640
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2049 | EPHB3 | EPH receptor B3 | NM_004443.4 | 100% | 100% | |
| 2 | mouse | 13845 | Ephb3 | Eph receptor B3 | NM_010143.1 | 88.2% | 96.1% | (many diffs) |
| 3 | mouse | 13845 | Ephb3 | Eph receptor B3 | XM_006521788.1 | 83.6% | 91% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3066
- ORF length:
- 2994
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccaga gcccgcccgc cgccgccgcc gtcgccgccg ccggggcttc 121 tgccgctgct ccctccgctg ctgctgctgc cgctgctgct gctgcccgcc ggctgccggg 181 cgctggaaga gaccctcatg gacacaaaat gggtaacatc tgagttggcg tggacatctc 241 atccagaaag tgggtgggaa gaggtgagtg gctacgatga ggccatgaat cccatccgca 301 cataccaggt gtgtaatgtg cgcgagtcaa gccagaacaa ctggcttcgc acggggttca 361 tctggcggcg ggatgtgcag cgggtctacg tggagctcaa gttcactgtg cgtgactgca 421 acagcatccc caacatcccc ggctcctgca aggagacctt caacctcttc tactacgagg 481 ctgacagcga tgtggcctca gcctcctccc ccttctggat ggagaacccc tacgtgaaag 541 tggacaccat tgcacccgat gagagcttct cgcggctgga tgccggccgt gtcaacacca 601 aggtgcgcag ctttgggcca ctttccaagg ctggcttcta cctggccttc caggaccagg 661 gcgcctgcat gtcgctcatc tccgtgcgcg ccttctacaa gaagtgtgca tccaccaccg 721 caggcttcgc actcttcccc gagaccctca ctggggcgga gcccacctcg ctggtcattg 781 ctcctggcac ctgcatccct aacgccgtgg aggtgtcggt gccactcaag ctctactgca 841 acggcgatgg ggagtggatg gtgcctgtgg gtgcctgcac ctgtgccacc ggccatgagc 901 cagctgccaa ggagtcccag tgccgcccct gtccccctgg gagctacaag gcgaagcagg 961 gagaggggcc ctgcctccca tgtcccccca acagccgtac cacctcccca gccgccagca 1021 tctgcacctg ccacaataac ttctaccgtg cagactcgga ctctgcggac agtgcctgta 1081 ccaccgtgcc atctccaccc cgaggtgtga tctccaatgt gaatgaaacc tcactgatcc 1141 tcgagtggag tgagccccgg gacctgggtg gccgggatga cctcctgtac aatgtcatct 1201 gcaagaagtg ccatggggct ggaggggcct cagcctgctc acgctgtgat gacaacgtgg 1261 agtttgtgcc tcggcagctg ggcctgacgg agcgccgggt ccacatcagc catctgctgg 1321 cccacacgcg ctacaccttt gaggtgcagg cggtcaacgg tgtctcgggc aagagccctc 1381 tgccgcctcg ttatgcggcc gtgaatatca ccacaaacca ggctgccccg tctgaagtgc 1441 ccacactacg cctgcacagc agctcaggca gcagcctcac cctatcctgg gcacccccag 1501 agcggcccaa cggagtcatc ctggactacg agatgaagta ctttgagaag agcgagggca 1561 tcgcctccac agtgaccagc cagatgaact ccgtgcagct ggacgggctt cggcctgacg 1621 cccgctatgt ggtccaggtc cgtgcccgca cagtagctgg ctatgggcag tacagccgcc 1681 ctgccgagtt tgagaccaca agtgagagag gctctggggc ccagcagctc caggagcagc 1741 ttcccctcat cgtgggctcc gctacagctg ggcttgtctt cgtggtggct gtcgtggtca 1801 tcgctatcgt ctgcctcagg aagcagcgac acggctctga ttcggagtac acggagaagc 1861 tgcagcagta cattgctcct ggaatgaagg tttatattga cccttttacc tacgaggacc 1921 ctaatgaggc tgttcgggag tttgccaagg agatcgacgt gtcctgcgtc aagatcgagg 1981 aggtgatcgg agctggggaa tttggggaag tgtgccgtgg tcgactgaaa cagcctggcc 2041 gccgagaggt gtttgtggcc atcaagacgc tgaaggtggg ctacaccgag aggcagcggc 2101 gggacttcct aagcgaggcc tccatcatgg gtcagtttga tcaccccaat ataatccggc 2161 tcgagggcgt ggtcaccaaa agtcggccag ttatgatcct cactgagttc atggaaaact 2221 gcgccctgga ctccttcctc cggctcaacg atgggcagtt cacggtcatc cagctggtgg 2281 gcatgttgcg gggcattgct gccggcatga agtacctgtc cgagatgaac tatgtgcacc 2341 gcgacctggc tgctcgcaac atccttgtca acagcaacct ggtctgcaaa gtctcagact 2401 ttggcctctc ccgcttcctg gaggatgacc cctccgatcc tacctacacc agttccctgg 2461 gcgggaagat ccccatccgc tggactgccc cagaggccat agcctatcgg aagttcactt 2521 ctgctagtga tgtctggagc tacggaattg tcatgtggga ggtcatgagc tatggagagc 2581 gaccctactg ggacatgagc aaccaggatg tcatcaatgc cgtggagcag gattaccggc 2641 tgccaccacc catggactgt cccacagcac tgcaccagct catgctggac tgctgggtgc 2701 gggaccggaa ccTCAGGCCC AAATTCTCCC AGATTGTCAA TACCCTGGAC AAGCTCATCC 2761 GCAATGCTGC CAGCCTCAAG GTCATTGCCA GCGCTCAGTC TGGCATGTCA CAGCCCCTCC 2821 TGGACCGCAC GGTCCCAGAT TACACAACCT TCACGACAGT TGGTGATTGG CTGGATGCCA 2881 TCAAGATGGG GCGGTACAAG GAGAGCTTCG TCAGTGCGGG GTTTGCATCT TTTGACCTGG 2941 TGGCCCAGAT GACGGCAGAA GACCTGCTCC GTATTGGGGT CACCCTGGCC GGCCACCAGA 3001 AGAAGATCCT GAGCAGTATC CAGGACATGC GGCTGCAGAT GAACCAGACG CTGCCTGTGC 3061 AGGTCTAGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 3121 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 3181 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTTTTT CACCTTCCTT GATTGAAGAC 3241 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt