Construct: ORF TRCN0000488647
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019174.1_s317c1
- DNA Barcode:
- ATCATTGACACGGGTTCCCTAGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- P2RY11 (5032)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488647
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5032 | P2RY11 | purinergic receptor P2Y11 | NM_002566.5 | 99.9% | 100% | 237C>T |
| 2 | human | 692312 | PPAN-P2RY11 | PPAN-P2RY11 readthrough | NM_001040664.3 | 46.8% | 46.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1197
- ORF length:
- 1122
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggca gccaacgtct cgggtgccaa gtcctgccct gccaacttct 121 tggcagctgc cgacgacaaa ctcagtgggt tccaggggga cttcctgtgg cccatactgg 181 tggttgagtt cctggtggcc gtggccagca atggcctggc cctgtaccgc ttcagcatcc 241 ggaagcagcg cccatggcac cccgccgtgg tcttctctgt ccagctggca gtcagcgacc 301 tgctctgcgc tctgacgctg cccccgctgg ccgcctacct ctatcccccc aagcactggc 361 gctatgggga ggccgcgtgc cgcctggagc gcttcctctt cacctgcaac ctgctgggca 421 gcgtcatctt catcacctgc atcagcctca accgctacct gggcatcgtg caccccttct 481 tcgcccgaag ccacctgcga cccaagcacg cctgggccgt gagcgctgcc ggctgggtcc 541 tggccgccct gctggccatg cccacactca gcttctccca cctgaagagg ccgcagcagg 601 gggcgggcaa ctgcagcgtg gccaggcccg aggcctgcat caagtgtctg gggacagcag 661 accacgggct ggcggcctac agagcgtata gcctggtgct ggcggggttg ggctgcggcc 721 tgccgctgct gctcacgctg gcagcctacg gcgccctcgg gcgggccgtg ctacgcagcc 781 caggcatgac tgtggccgag aagctgcgtg tggcagcgtt ggtggccagt ggtgtggccc 841 tctacgccag ctcctatgtg ccctaccaca tcatgcgggt gctcaacgtg gatgctcggc 901 ggcgctggag cacccgctgc ccgagctttg cagacatagc ccaggccaca gcagccctgg 961 agctgggGCC CTACGTGGGC TACCAGGTGA TGCGGGGCCT CATGCCCCTG GCCTTCTGTG 1021 TCCACCCTCT ACTCTACATG GCCGCAGTGC CCAGCCTGGG CTGCTGCTGC CGACACTGCC 1081 CCGGCTACAG GGACAGCTGG AACCCAGAGG ACGCCAAGAG CACTGGCCAA GCCCTGCCCC 1141 TCAATGCCAC AGCCGCCCCT AAACCGTCAG AGCCCCAGTC CCGTGAGCTG AGCCAAGACC 1201 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1261 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1321 ATATATCTTG TGGAAAGGAC GAATCATTGA CACGGGTTCC CTAGTTACGC GTTAAGTCga 1381 caatcaacct ctggattaca aaatttgtga aagatt