Construct: ORF TRCN0000488656
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019510.1_s317c1
- DNA Barcode:
- CAAGTTTTGCAATCCGCAATGTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TESK2 (10420)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488656
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10420 | TESK2 | testis associated actin rem... | NM_007170.3 | 94.7% | 94.7% | 793_879del;1711_1713delGGG |
| 2 | human | 10420 | TESK2 | testis associated actin rem... | NM_001320800.2 | 80.2% | 80.2% | 0_1ins249;544_630del;1462_1464delGGG |
| 3 | mouse | 230661 | Tesk2 | testis-specific kinase 2 | NM_146151.4 | 82.1% | 82.8% | (many diffs) |
| 4 | mouse | 230661 | Tesk2 | testis-specific kinase 2 | XM_006503008.3 | 64.3% | 64.2% | (many diffs) |
| 5 | mouse | 230661 | Tesk2 | testis-specific kinase 2 | XR_001784124.1 | 41.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 1713
- ORF length:
- 1623
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcacctct agacagacta tggatcggag caaacggaat tcaattgcag 121 gatttcctcc acgtgtggag cgtcttgaag agtttgaagg aggtggtgga ggagaaggaa 181 atgtgagcca ggtgggaaga gtttggccat cttcgtatcg agctcttata agtgcctttt 241 ccagactgac gcgtttggat gatttcacct gtgaaaaaat agggtctggc ttcttttctg 301 aagtgttcaa ggtacgacac cgagcttctg gtcaggtgat ggctcttaag atgaacacat 361 tgagcagtaa ccgggcaaac atgctgaaag aagtacagct catgaataga ctctcccatc 421 ccaacatcct taggttcatg ggtgtatgtg ttcatcaagg acaattgcat gcacttacag 481 agtatatcaa ctccgggaac ctggaacagt tgctagacag taacctgcat ttgccttgga 541 ctgtgagggt aaaactggcc tatgacatag cagtgggcct cagctacctt cacttcaaag 601 gcatttttca tcgggacctc acatctaaga actgcctgat aaagagggat gagaatggtt 661 actctgcagt ggtagctgac tttggcctgg ctgagaagat ccccgatgtc agcatgggga 721 gtgagaagct ggccgtggtg ggttccccat tctggatggc acctgaggtt ctccgagatg 781 agccctataa tgaaaaggca gatgtgttct cttatggtat catcctctgc gagatcatcg 841 cccgcatcca ggccgatccg gactatcttc cccgcacaga gatggatccc aaactgcgcc 901 catcttttgt ggagattggg aagaccctgg aggaaattct gagccgccta caggaagaag 961 agcaggagag ggataggaag ctgcagccca cagccagggg actcttggag aaagcacctg 1021 gggtgaagcg actaagctca ctggatgaca agatccccca caagtcacca tgcccaagac 1081 gtaccatctg gctgtctcga agccagtcag atatcttttc ccgtaagccc ccacgtacag 1141 tgagtgtctt ggacccatac taccggccac gagatggtgc tgcccgcacc cccaaagtca 1201 acccttttag tgctcgccag gacctcatgg ggggcaagat caagtttttt gacctgccca 1261 gcaagtctgt catctctctg gtatttgacc tggatgcacc agggcccgga actatgcccc 1321 tggctgactg gcaggagccc ctggccccac ctattcgccg gtggcgttcc ttgcctggtt 1381 cgccTGAGTT CTTGCATCAA GAGGCTTGTC CATTTGTGGG CCGGGAAGAA TCGCTATCTG 1441 ATGGGCCCCC ACCACGCCTA AGTAGTCTCA AGTACAGAGT TAAAGAGATC CCACCATTCC 1501 GGGCATCTGC CCTACCAGCT GCTCAAGCCC ATGAGGCTAT GGACTGCTCC ATTCTCCAGG 1561 AAGAAAATGG TTTTGGGTCC AGGCCCCAGG GGACCAGTCC ATGCCCTGCG GGTGCTTCTG 1621 AGGAGATGGA GGTAGAAGAA AGGCCAGCAG GCTCAACTCC AGCCACCTTC TCCACCTCAG 1681 GCATAGGCCT GCAAACCCAG GGAAAGCAGG ATAAGGGTGG GCGCGCCGAC CCAGCTTTCT 1741 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1801 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1861 GTGGAAAGGA CGACAAGTTT TGCAATCCGC AATGTCCACG CGTTAAGTCg acaatcaacc 1921 tctggattac aaaatttgtg aaagatt