Construct: ORF TRCN0000488669
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021757.1_s317c1
- DNA Barcode:
- ATTGGTAGACGCAATATGCTTGCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PLCG2 (5336)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488669
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5336 | PLCG2 | phospholipase C gamma 2 | NM_002661.5 | 99.9% | 100% | 174T>C;1188C>G;3093T>C |
| 2 | mouse | 234779 | Plcg2 | phospholipase C, gamma 2 | NM_172285.2 | 86.5% | 94.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3864
- ORF length:
- 3795
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gtccaccacg gtcaatgtag attcccttgc ggaatatgag aagagccaga 121 tcaagagagc cctggagctg gggacggtga tgactgtgtt cagcttccgc aagtccaccc 181 ccgagcggag aaccgtccag gtgatcatgg agacgcggca ggtggcctgg agcaagaccg 241 ccgacaagat cgagggcttc ttggatatca tggaaataaa agaaatccgc ccagggaaga 301 actccaaaga tttcgagcga gcaaaagcag ttcgccagaa agaagactgc tgcttcacca 361 tcctatatgg cactcagttc gtcctcagca cgctcagctt ggcagctgac tctaaagagg 421 atgcagttaa ctggctctct ggcttgaaaa tcttacacca ggaagcgatg aatgcgtcca 481 cgcccaccat tatcgagagt tggctgagaa agcagatata ttctgtggat caaaccagaa 541 gaaacagcat cagtctccga gagttgaaga ccatcttgcc cctgatcaac tttaaagtga 601 gcagtgccaa gttccttaaa gataagtttg tggaaatagg agcacacaaa gatgagctca 661 gctttgaaca gttccatctc ttctataaaa aacttatgtt tgaacagcaa aaatcgattc 721 tcgatgaatt caaaaaggat tcgtccgtgt tcatcctggg gaacactgac aggccggatg 781 cctctgctgt ttacctgcat gacttccaga ggtttctcat acatgaacag caggagcatt 841 gggctcagga tctgaacaaa gtccgtgagc ggatgacaaa gttcattgat gacaccatgc 901 gtgaaactgc tgagcctttc ttgtttgtgg atgagttcct cacgtacctg ttttcacgag 961 aaaacagcat ctgggatgag aagtatgacg cggtggacat gcaggacatg aacaaccccc 1021 tgtctcatta ctggatctcc tcgtcacata acacgtacct tacaggtgac cagctgcgga 1081 gcgagtcgtc cccagaagct tacatccgct gcctgcgcat gggctgtcgc tgcattgaac 1141 tggactgctg ggacgggccc gatgggaagc cggtcatcta ccatggctgg acgcggacta 1201 ccaagatcaa gtttgacgac gtcgtgcagg ccatcaaaga ccacgccttt gttacgtcga 1261 gcttcccagt gatcctgtcc atcgaggagc actgcagcgt ggagcaacag cgtcacatgg 1321 ccaaggcctt caaggaagta tttggcgacc tgctgttgac gaagcccacg gaggccagtg 1381 ctgaccagct gccctcgccc agccagctgc gggagaagat catcatcaag cataagaagc 1441 tgggcccccg aggcgatgtg gatgtcaaca tggaggacaa gaaggacgaa cacaagcaac 1501 agggggagct gtacatgtgg gattccattg accagaaatg gactcggcac tactgcgcca 1561 ttgccgatgc caagctgtcc ttcagtgatg acattgaaca gactatggag gaggaagtgc 1621 cccaggatat accccctaca gaactacatt ttggggagaa atggttccac aagaaggtgg 1681 agaagaggac gagtgccgag aagttgctgc aggaatactg catggagacg gggggcaagg 1741 atggcacctt cctggttcgg gagagcgaga ccttccccaa tgactacacc ctgtccttct 1801 ggcggtcagg ccgggtccag cactgccgga tccgctccac catggagggc gggaccctga 1861 aatactactt gactgacaac ctcaccttca gcagcatcta tgccctcatc cagcactacc 1921 gcgagacgca cctgcgctgc gccgagttcg agctgcggct cacggaccct gtgcccaacc 1981 ccaaccccca cgagtccaag ccgtggtact atgacagcct gagccgcgga gaggcagagg 2041 acatgctgat gaggattccc cgggacgggg ccttcctgat ccggaagcga gaggggagcg 2101 actcctatgc catcaccttc agggctaggg gcaaggtaaa gcattgtcgc atcaaccggg 2161 acggccggca ctttgtgctg gggacctccg cctattttga gagtctggtg gagctcgtca 2221 gttactacga gaagcattca ctctaccgaa agatgagact gcgctacccc gtgacccccg 2281 agctcctgga gcgctacaat atggaaagag atataaactc cctctacgac gtcagcagaa 2341 tgtatgtgga tcccagtgaa atcaatccgt ccatgcctca gagaaccgtg aaagctctgt 2401 atgactacaa agccaagcga agcgatgagc tgagcttctg ccgtggtgcc ctcatccaca 2461 atgtctccaa ggagcccggg ggctggtgga aaggagacta tggaaccagg atccagcagt 2521 acttcccatc caactacgtc gaggacatct caactgcaga cttcgaggag ctagaaaagc 2581 agattattga agacaatccc ttagggtctc tttgcagagg aatattggac ctcaatacct 2641 ataacgtcgt gaaagcccct cagggaaaaa accagaagtc ctttgtcttc atcctggagc 2701 ccaagcagca gggcgatcct ccggtggagt ttgccacaga cagggtggag gagctctttg 2761 agtggtttca gagcatccga gagatcacct ggaagattga caccaaggag aacaacatga 2821 agtactggga gaagaaccag tccatcgcca tcgagctctc tgacctggtt gtctactgca 2881 aaccaaccag caaaaccaag gacaacttag aaaatcctga cttccgagaa atccgctcct 2941 ttgtggagac gaaggctgac agcatcatca gacagaagcc cgtcgacctc ctgaagtaca 3001 atcaaaaggg cctgacccgc gtctacccaa agggacaaag agttgactct tcaaactacg 3061 accccttccg cctctggctg tgcggttctc agatggtggc actcaatttc cagacggcag 3121 ataagtacat gcagatgaat cacgcattgt tttctctcaa cgggcgcacg ggctacgttc 3181 tgcagcctga gagcatgagg acagagaaat atgacccgat gccacccgag tcccagagga 3241 agatcctgat gacgctgaca gtcaaggttc tcggtgctcg ccatctcccc aaacttggac 3301 gaagtattgc ctgtcccttt gtagaagtgg agatctgtgg agccgagtat gacaacaaca 3361 agttcaagac gacggttgtg aatgataatg gcctcagccc tatctgggct ccaacacagg 3421 agaaggtgac atttgaaatt tatgacccaa acctggcatt tctgcgcttt gtggtttatg 3481 aagaagatat gttcagcgat cccaactttc ttgctcatgc cacttacccc attaaagcag 3541 tcaaatcagg attcaggtcc gttcctctga agaatgggta cagcgaggac atagagctgg 3601 cttcccTCCT GGTTTTCTGT GAGATGCGGC CAGTCCTGGA GAGCGAAGAG GAACTTTACT 3661 CCTCCTGTCG CCAGCTGAGG AGGCGGCAAG AAGAACTGAA CAACCAGCTC TTTCTGTATG 3721 ACACACACCA GAACTTGCGC AATGCCAACC GGGATGCCCT GGTTAAAGAG TTCAGTGTTA 3781 ATGAGAACCA GCTCCAGCTG TACCAGGAGA AATGCAACAA GAGGTTAAGA GAGAAGAGAG 3841 TCAGCAACAG CAAGTTTTAC TCATAGAACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 3901 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 3961 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATTGGTAG 4021 ACGCAATATG CTTGCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 4081 aagatt