Construct: ORF TRCN0000488673
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021274.1_s317c1
- DNA Barcode:
- GGTCAATATGAAATTTCAAAGAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HSD17B12 (51144)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488673
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51144 | HSD17B12 | hydroxysteroid 17-beta dehy... | NM_016142.3 | 30.7% | 30.1% | (many diffs) |
| 2 | human | 51144 | HSD17B12 | hydroxysteroid 17-beta dehy... | XM_017017881.1 | 18.6% | 13.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 363
- ORF length:
- 294
- Sequence:
-
1 tcttcCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 CAGGCACCAT GGAGAGCGCT CTCCCCGCCG CCGGCTTCCT GTACTGGGTC GGCGCGGGCA 121 CCGTGGCCTA CCTAGCCCTG CGTATTTCGT ACTCGCTCTT CACGGCCCTC CGGGTCTGGG 181 GAGTGGGGAA TGAGGCGGGG GTCGGCCCGG GGCTCGGAGA ATGGGCAGTT GTCACAGGTA 241 GTACTGATGG AATTGGAAAA TCATATGCAG AAGAGTTAGC AAAGCATGGA ATGAAGGTTG 301 TCCTTATCAG CAGATCAAAG GATAAACTTG ACCAGGTTTC CAGTGAAATA AGCAATTATA 361 CTTAGAACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 421 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 481 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGGTCAATAT GAAATTTCAA AGAATACGCG 541 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt