Construct: ORF TRCN0000488700
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021658.1_s317c1
- DNA Barcode:
- CGGATCTATTCCTGGGACATGGAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PRKAB2 (5565)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488700
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5565 | PRKAB2 | protein kinase AMP-activate... | NM_005399.5 | 100% | 100% | |
| 2 | human | 5565 | PRKAB2 | protein kinase AMP-activate... | XM_011509729.2 | 88.8% | 91.5% | 742_778del;795_796ins58 |
| 3 | human | 5565 | PRKAB2 | protein kinase AMP-activate... | XM_017001697.2 | 72.4% | 54.2% | 413_414ins121;591_592ins104 |
| 4 | human | 5565 | PRKAB2 | protein kinase AMP-activate... | NR_103871.2 | 12.1% | 1_65del;219_220ins167;715_5176del | |
| 5 | human | 5565 | PRKAB2 | protein kinase AMP-activate... | XR_001737291.2 | 12.1% | 1_961del;1701_2185del;2263_6724del | |
| 6 | human | 5565 | PRKAB2 | protein kinase AMP-activate... | XR_001737292.2 | 11.2% | (many diffs) | |
| 7 | human | 5565 | PRKAB2 | protein kinase AMP-activate... | NR_103870.2 | 9.8% | (many diffs) | |
| 8 | mouse | 108097 | Prkab2 | protein kinase, AMP-activat... | NM_182997.2 | 90.9% | 97% | (many diffs) |
| 9 | mouse | 108097 | Prkab2 | protein kinase, AMP-activat... | XM_006500840.1 | 90.9% | 97% | (many diffs) |
| 10 | mouse | 108097 | Prkab2 | protein kinase, AMP-activat... | XM_011239982.2 | 90.9% | 97% | (many diffs) |
| 11 | mouse | 108097 | Prkab2 | protein kinase, AMP-activat... | XM_006500841.1 | 75.5% | 79.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 885
- ORF length:
- 816
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gggaaacacc accagcgacc gggtgtccgg ggagcgccac ggcgccaagg 121 ctgcacgctc cgagggcgca ggcggccatg ccccggggaa ggagcacaag atcatggtgg 181 ggagtacgga cgaccccagc gtgttcagcc tccctgactc caagctccct ggggacaaag 241 agtttgtatc atggcagcag gatttggagg actccgtaaa gcccacacag caggcccggc 301 ccactgttat ccgctggtct gaaggaggca aggaggtctt catctctggg tccttcaaca 361 attggagcac caagattcca ctgattaaga gccataatga ctttgttgcc atcctggacc 421 tccctgaggg agagcaccaa tacaagttct ttgtggatgg acagtgggtt catgatccat 481 cagagcctgt ggttaccagt cagcttGGCA CAATTAACAA TTTGATCCAT GTCAAGAAAT 541 CTGATTTTGA GGTGTTCGAT GCTTTAAAGT TAGATTCTAT GGAAAGTTCT GAGACATCTT 601 GTAGAGACCT TTCCAGCTCA CCCCCAGGGC CTTATGGTCA AGAAATGTAT GCGTTTCGAT 661 CTGAGGAAAG ATTCAAATCC CCACCCATCC TTCCTCCTCA TCTACTTCAA GTTATTCTTA 721 ACAAAGACAC TAATATTTCT TGTGACCCAG CCTTACTCCC TGAGCCCAAC CATGTTATGC 781 TGAACCATCT CTATGCATTG TCCATTAAGG ACAGTGTGAT GGTCCTTAGC GCAACCCATC 841 GCTACAAGAA GAAGTATGTT ACTACTCTGC TATACAAGCC CATTTAGAAC CCAGCTTTCT 901 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 961 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1021 GTGGAAAGGA CGACGGATCT ATTCCTGGGA CATGGACACG CGTTAAGTCg acaatcaacc 1081 tctggattac aaaatttgtg aaagatt