Construct: ORF TRCN0000488722

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020223.1_s317c1
DNA Barcode:
TGATGCAACTCCCATTCACCCTGT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
MAPK3 (5595)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488722
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5595 MAPK3 mitogen-activated protein k... NM_002746.3 100% 100%
2 human 5595 MAPK3 mitogen-activated protein k... NM_001040056.3 92.8% 88.1% (many diffs)
3 human 5595 MAPK3 mitogen-activated protein k... NM_001109891.1 88.3% 88.3% 774_775ins132
4 human 5595 MAPK3 mitogen-activated protein k... XR_243293.1 59% 1_100del;1238_1927del
5 mouse 26417 Mapk3 mitogen-activated protein k... NM_011952.2 90% 96.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1209
ORF length:
1137
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggcggcg gcggcggctc aggggggcgg gggcggggag ccccgtagaa
121ccgagggggt cggcccgggg gtcccggggg aggtggagat ggtgaagggg cagccgttcg
181acgtgggccc gcgctacacg cagttgcagt acatcggcga gggcgcgtac ggcatggtca
241gctcggccta tgaccacgtg cgcaagactc gcgtggccat caagaagatc agccccttcg
301aacatcagac ctactgccag cgcacgctcc gggagatcca gatcctgctg cgcttccgcc
361atgagaatgt catcggcatc cgagacattc tgcgggcgtc caccctggaa gccatgagag
421atgtctacat tgtgcaggac ctgatggaga ctgacctgta caagttgctg aaaagccagc
481agctgagcaa tgaccatatc tgctacttcc tctaccagat cctgcggggc ctcaagtaca
541tccactccgc caacgtgctc caccgagatc taaagccctc caacctgctc atcaacacca
601cctgcgacct taagatttgt gatttcggcc tggcccggat tgccgatcct gagcatgacc
661acaccggctt cctgacggag tatgtggcta cgcgctggta ccgggcccca gagatcatgc
721tgaactccaa gggctatacc aagtccatcg acatctggtc tgtgggctgc attctggctg
781agatgctctc taaccggccc atcttccctg gcaagcacta cctggatcag ctcaaccaca
841ttctgggcat cctgggctcc ccatcccagg aggacctgaa ttgtatcatc aacatgaagg
901cccgaaacta ccTACAGTCT CTGCCCTCCA AGACCAAGGT GGCTTGGGCC AAGCTTTTCC
961CCAAGTCAGA CTCCAAAGCC CTTGACCTGC TGGACCGGAT GTTAACCTTT AACCCCAATA
1021AACGGATCAC AGTGGAGGAA GCGCTGGCTC ACCCCTACCT GGAGCAGTAC TATGACCCGA
1081CGGATGAGCC AGTGGCCGAG GAGCCCTTCA CCTTCGCCAT GGAGCTGGAT GACCTACCTA
1141AGGAGCGGCT GAAGGAGCTC ATCTTCCAGG AGACAGCACG CTTCCAGCCC GGAGTGCTGG
1201AGGCCCCCTG AGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1261ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1321CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATGA TGCAACTCCC ATTCACCCTG
1381TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)