Construct: ORF TRCN0000488781
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020098.1_s317c1
- DNA Barcode:
- AAATAATTTCAACGAACCACTAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UHMK1 (127933)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488781
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 127933 | UHMK1 | U2AF homology motif kinase 1 | NM_175866.5 | 100% | 100% | |
| 2 | human | 127933 | UHMK1 | U2AF homology motif kinase 1 | NM_144624.2 | 81.9% | 81.3% | (many diffs) |
| 3 | human | 127933 | UHMK1 | U2AF homology motif kinase 1 | NM_001184763.1 | 80.8% | 79.2% | (many diffs) |
| 4 | mouse | 16589 | Uhmk1 | U2AF homology motif (UHM) k... | NM_010633.3 | 92.9% | 99.2% | (many diffs) |
| 5 | mouse | 16589 | Uhmk1 | U2AF homology motif (UHM) k... | XM_006496682.3 | 75.9% | 80.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 100
- ORF end:
- 1357
- ORF length:
- 1257
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggccgccccc ttcaccctct agaacaccga tggcgggatc cggctgcgcc 121 tggggcgcgg agccgccgcg ttttctggag gccttcgggc ggctgtggca ggtacagagc 181 cgtctgggta gcggctcctc cgcctcggtg tatcgggttc gctgctgcgg caaccctggc 241 tcgccccccg gcgccctcaa gcagttcttg ccgccaggaa ccaccggggc tgcggcctct 301 gccgccgagt atggtttccg caaagagagg gcggcgctgg aacagttgca gggtcacaga 361 aacatcgtga ctttgtatgg agtgtttaca atccactttt ctccaaatgt gccatcacgc 421 tgtctgttgc ttgaactcct ggatgtcagt gtttcggaat tgctcttata ttccagtcac 481 cagggttgtt ccatgtggat gatacagcat tgtgcccgag atgttttgga ggcccttgct 541 tttcttcatc atgagggcta tgtccatgcg gacctcaaac cacgtaacat attgtggagt 601 gcagagaatg aatgttttaa actcattgac tttggactta gcttcaaaga aggcaatcag 661 gatgtaaagt atattcagac agacgggtat cgggctccag aagcagaatt gcaaaattgc 721 ttggcccagg ctggcctgca gagtgataca gaatgtacct cagctgttga tctgtggagc 781 ctaggaatca ttttactgga aatgttctca ggaatgaaac tgaaacatac agtcagatct 841 caggaatgga aggcaaacag ttctgctatt attgatcaca tatttgccag taaagcagtg 901 gtgaatgccg caattccagc ctatcaccta agagacctta tcaaaagcat gcttcatgat 961 gatccaagca gaagaattcc tgctgaaatg gcattgtgca gcccattctt tagcattcct 1021 tttgcccctc atattgaaga tctggtcatg cttccCACTC CAGTGCTAAG ACTGCTGAAT 1081 GTGCTGGATG ATGATTATCT TGAGAATGAA GAGGAATATG AAGATGTTGT AGAAGATGTA 1141 AAAGAGGAGT GTCAAAAATA TGGACCAGTG GTATCTCTAC TTGTTCCAAA GGAAAATCCT 1201 GGCAGAGGAC AAGTCTTTGT TGAGTATGCA AATGCTGGTG ATTCCAAAGC TGCGCAGAAA 1261 TTACTGACTG GAAGGATGTT TGATGGGAAG TTTGTTGTGG CTACATTCTA CCCGCTGAGT 1321 GCCTACAAGA GGGGATATCT GTATCAAACC TTGCTTAAGG GTGGGCGCGC CGACCCAGCT 1381 TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT 1441 TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA 1501 TCTTGTGGAA AGGACGAAAA TAATTTCAAC GAACCACTAA AACGCGTTAA GTCgacaatc 1561 aacctctgga ttacaaaatt tgtgaaagat t