Construct: ORF TRCN0000488813
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019924.1_s317c1
- DNA Barcode:
- TCAGTTCCATTGTAACGAGTCCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RSKR (124923)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488813
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 124923 | RSKR | ribosomal protein S6 kinase... | NM_001174103.2 | 66.1% | 66% | 810_811insGGTGA;812_815delCCCC;822_1230delinsG |
| 2 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XM_006532965.3 | 56.4% | 54.8% | (many diffs) |
| 3 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | NM_001287206.1 | 56.2% | 54.6% | (many diffs) |
| 4 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XR_388406.3 | 55.8% | (many diffs) | |
| 5 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XM_006532966.3 | 51.4% | 49.5% | (many diffs) |
| 6 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XR_388407.3 | 48.7% | (many diffs) | |
| 7 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XM_006532967.3 | 45.2% | 42.9% | (many diffs) |
| 8 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XM_006532968.3 | 32.5% | 33.9% | (many diffs) |
| 9 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XR_388408.3 | 20.5% | (many diffs) | |
| 10 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XR_001779956.1 | 15.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 894
- ORF length:
- 825
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gggagcagta agctgtcggc aggggcagca cacccagcag ggggaacaca 121 cccgggtggc tgtccctcac aagcagggtg gcaacatccg gggtccctgg gcccgaggct 181 ggaagagcct ctggacaggt ttgggaacca tcaggtcaga tctggaagaa ctctgggaac 241 tacgggggca ccactatctg caccaggaat ccctaaagcc agccccagta ctggtagaga 301 agcctctgcc agagtggcca gtgcctcagt tcatcaacct ctttctacca gagtttccca 361 ttaggcccat tagggggcag cagcagctga agattttagg cctcgtggct aaaggctcct 421 ttggaactgt cctcaaggtg ctagattgca cccagaaagc tgtatttgca gtgaaggtgg 481 tgcccaaggt aaaggtccta cagagggata ccgtgaggca gtgcaaagag gaggttagca 541 tccagcgaca gatcaaccat ccctttgtac acagcttGGG GGACAGCTGG CAGGGAAAAC 601 GGCACCTTTT CATTATGTGT AGCTACTGCA GCACAGATCT GTACTCCCTT TGGTCGGCTG 661 TTGGCTGCTT TCCTGAGGCT TCCATCCGTC TCTTTGCTGC CGAGTTGGTG CTGGTACTGT 721 GTTATCTCCA TGACTTGGGC ATCATGCATC GAGATGTGAA GATGGAGAAT ATTCTTCTAG 781 ATGAACGAGG CCATCTGAAA CTGACAGACT TTGGTCTGTC CCGCCACGTG CCCCAGGGAG 841 CTCAAGCCTA CACTATCTGT GGCACTCTTC AGTACATGGG TGAGAGAGGT GACCCAGCTT 901 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 961 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1021 CTTGTGGAAA GGACGATCAG TTCCATTGTA ACGAGTCCCG ACGCGTTAAG TCgacaatca 1081 acctctggat tacaaaattt gtgaaagatt