Construct: ORF TRCN0000488827
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021441.1_s317c1
- DNA Barcode:
- TCGACCGCCCCCAGGTCGCTGAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- COMT (1312)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488827
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1312 | COMT | catechol-O-methyltransferase | NM_007310.3 | 100% | 100% | |
| 2 | human | 1312 | COMT | catechol-O-methyltransferase | NM_000754.4 | 81.5% | 81.5% | 1_150del |
| 3 | human | 1312 | COMT | catechol-O-methyltransferase | NM_001135161.2 | 81.5% | 81.5% | 1_150del |
| 4 | human | 1312 | COMT | catechol-O-methyltransferase | NM_001135162.2 | 81.5% | 81.5% | 1_150del |
| 5 | human | 1312 | COMT | catechol-O-methyltransferase | NM_001362828.2 | 81.5% | 81.5% | 1_150del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 732
- ORF length:
- 663
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcat gggtgacacc aaggagcagc gcatcctgaa ccacgtgctg cagcatgcgg 121 agcccgggaa cgcacagagc gtgctggagg ccattgacac ctactgcgag cagaaggagt 181 gggccatgaa cgtgggcgac aagaaaggca agatcgtgga cgccgtgatt caggagcacc 241 agccctccgt gctgctggag ctgggggcct actgtggcta ctcagctgtg cgcatggccc 301 gcctgctgtc accaggggcg aggctcatca ccatcgagat caaccccgac tgtgccgcca 361 tcacccagcg gatggtggat ttcgctggcg tgaaggacaa ggtcaccctt gtggttggag 421 cgtcccagga catcatcccc cagctgaaga agaagtatga tgtggacaca ctggacatgg 481 tcttcctcga ccactggaag gaccggtacc tgccggacac gcttctcttG GAGGAATGTG 541 GCCTGCTGCG GAAGGGGACA GTGCTACTGG CTGACAACGT GATCTGCCCA GGTGCGCCAG 601 ACTTCCTAGC ACACGTGCGC GGGAGCAGCT GCTTTGAGTG CACACACTAC CAATCGTTCC 661 TGGAATACAG GGAGGTGGTG GACGGCCTGG AGAAGGCCAT CTACAAGGGC CCAGGCAGCG 721 AAGCAGGGCC CTGAGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 781 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 841 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TCGACCGCCC CCAGGTCGCT 901 GAGTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt