Construct: ORF TRCN0000488846
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020057.1_s317c1
- DNA Barcode:
- CTAGTCTAGACTTTAAATAGCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HDAC1 (3065)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488846
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3065 | HDAC1 | histone deacetylase 1 | NM_004964.3 | 99.9% | 99.7% | 1446_1447insG |
| 2 | human | 3065 | HDAC1 | histone deacetylase 1 | XM_011541309.2 | 59.9% | 59.8% | 0_1ins579;867_868insG |
| 3 | mouse | 433759 | Hdac1 | histone deacetylase 1 | NM_008228.2 | 90.3% | 99.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1518
- ORF length:
- 1449
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcgcagacg cagggcaccc ggaggaaagt ctgttactac tacgacgggg 121 atgttggaaa ttactattat ggacaaggcc acccaatgaa gcctcaccga atccgcatga 181 ctcataattt gctgctcaac tatggtctct accgaaaaat ggaaatctat cgccctcaca 241 aagccaatgc tgaggagatg accaagtacc acagcgatga ctacattaaa ttcttgcgct 301 ccatccgtcc agataacatg tcggagtaca gcaagcagat gcagagattc aacgttggtg 361 aggactgtcc agtattcgat ggcctgtttg agttctgtca gttgtctact ggtggttctg 421 tggcaagtgc tgtgaaactt aataagcagc agacggacat cgctgtgaat tgggctgggg 481 gcctgcacca tgcaaagaag tccgaggcat ctggcttctg ttacgtcaat gatatcgtct 541 tggccatcct ggaactgcta aagtatcacc agagggtgct gtacattgac attgatattc 601 accatggtga cggcgtggaa gaggccttct acaccacgga ccgggtcatg actgtgtcct 661 ttcataagta tggagagtac ttcccaggaa ctggggacct acgggatatc ggggctggca 721 aaggcaagta ttatgctgtt aactacccgc tccgagacgg gattgatgac gagtcctatg 781 aggccatttt caagccggtc atgtccaaag taatggagat gttccagcct agtgcggtgg 841 tcttacagtg tggctcagac tccctatctg gggatcggtt aggttgcttc aatctaacta 901 tcaaaggaca cgccaagtgt gtggaatttg tcaagagctt taacctgcct atgctgatgc 961 tgggaggcgg tggttacacc attcgtaacg ttgcccggtg ctggacatat gagacagctg 1021 tggccctgga tacggagatc cctaatgagc ttccatacaa tgactacttt gaatactttg 1081 gaccagattt caagctccac atcagtcctt ccaatatgac taaccagaac acgaatgagt 1141 acctggagaa gatcaaacag cgactgtttg agaaccttag aatgctgccg cacgcaccTG 1201 GGGTCCAAAT GCAGGCGATT CCTGAGGACG CCATCCCTGA GGAGAGTGGC GATGAGGACG 1261 AAGACGACCC TGACAAGCGC ATCTCGATCT GCTCCTCTGA CAAACGAATT GCCTGTGAGG 1321 AAGAGTTCTC CGATTCTGAA GAGGAGGGAG AGGGGGGCCG CAAGAACTCT TCCAACTTCA 1381 AAAAAGCCAA GAGAGTCAAA ACAGAGGATG AAAAAGAGAA AGACCCAGAG GAGAAGAAAG 1441 AAGTCACCGA AGAGGAGAAA ACCAAGGAGG AGAAGCCAGA AGCCAAAGGG GTCAAGGAGG 1501 AGGTCAAGTT GGCCGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1561 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1621 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTAGTCTAGA CTTTAAATAG 1681 CACCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt