Construct: ORF TRCN0000488853
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019382.1_s317c1
- DNA Barcode:
- CTGCACAACTGACACCTCTCTGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR87 (53836)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488853
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53836 | GPR87 | G protein-coupled receptor 87 | NM_023915.4 | 99.7% | 99.7% | 138G>A;537A>G;1074_1075insG |
| 2 | mouse | 84111 | Gpr87 | G protein-coupled receptor 87 | NM_001302203.1 | 86.5% | 91% | (many diffs) |
| 3 | mouse | 84111 | Gpr87 | G protein-coupled receptor 87 | XM_006502394.3 | 83.8% | 88.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1152
- ORF length:
- 1077
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgggg ttcaacttga cgcttgcaaa attaccaaat aacgagctgc 121 acggccaaga gagtcacaat tcaggcaaca ggagcgacgg gccaggaaag aacaccaccc 181 ttcacaatga atttgacaca attgtcttgc cagtgcttta tctcattata tttgtggcaa 241 gcatcttgct gaatggttta gcagtgtgga tcttcttcca cattaggaat aaaaccagct 301 tcatattcta tctcaaaaac atagtggttg cagacctcat aatgacgctg acatttccat 361 ttcgaatagt ccatgatgca ggatttggac cttggtactt caagtttatt ctctgcagat 421 acacttcagt tttgttttat gcaaacatgt atacttccat cgtgttcctt gggctgataa 481 gcattgatcg ctatctgaag gtggtcaagc catttgggga ctctcggatg tacagcataa 541 ccttcacgaa ggttttatct gtttgtgttt gggtgatcat ggctgttttg tctttgccaa 601 acatcatcct gacaaatggt cagccaacag aggacaatat ccatgactgc tcaaaactta 661 aaagtccttt gggggtcaaa tggcatacgg cagtcaccta tgtgaacagc tgcttgtttg 721 tggccgtgct ggtgattctg atcggatgtt acatagccat atccaggtac atccacaaaT 781 CCAGCAGGCA ATTCATAAGT CAGTCAAGCC GAAAGCGAAA ACATAACCAG AGCATCAGGG 841 TTGTTGTGGC TGTGTTTTTT ACCTGCTTTC TACCATATCA CTTGTGCAGA ATTCCTTTTA 901 CTTTTAGTCA CTTAGACAGG CTTTTAGATG AATCTGCACA AAAAATCCTA TATTACTGCA 961 AAGAAATTAC ACTTTTCTTG TCTGCGTGTA ATGTTTGCCT GGATCCAATA ATTTACTTTT 1021 TCATGTGTAG GTCATTTTCA AGAAGGCTGT TCAAAAAATC AAATATCAGA ACCAGGAGTG 1081 AAAGCATCAG ATCACTGCAA AGTGTGAGAA GATCGGAAGT TCGCATATAT TATGATTACA 1141 CTGATGTGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1201 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1261 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTGCAC AACTGACACC TCTCTGTTAC 1321 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt