Construct: ORF TRCN0000488870
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019181.1_s317c1
- DNA Barcode:
- GCACAGTCTCAGAGTGCCACAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAS2R3 (50831)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488870
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50831 | TAS2R3 | taste 2 receptor member 3 | NM_016943.2 | 99.8% | 100% | 807C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1023
- ORF length:
- 948
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgatg ggactcaccg agggggtgtt cctgattctg tctggcactc 121 agttcacact gggaattctg gtcaattgtt tcattgagtt ggtcaatggt agcagctggt 181 tcaagaccaa gagaatgtct ttgtctgact tcatcatcac caccctggca ctcttgagga 241 tcattctgct gtgtattatc ttgactgata gttttttaat agaattctct cccaacacac 301 atgattcagg gataataatg caaattattg atgtttcctg gacatttaca aaccatctga 361 gcatttggct tgccacctgt cttggtgtcc tctactgcct gaaaatcgcc agtttctctc 421 accccacatt cctctggctc aagtggagag tttctagggt gatggtatgg atgctgttgg 481 gtgcactgct cttatcctgt ggtagtaccg catctctgat caatgagttt aagctctatt 541 ctgtctttag gggaattgag gccaccagga atgtgactga acacttcaga aagaagagga 601 gtgagtatta tctgatccat gttcttggga ctctgtggta cctgcctccc ttaattgtgt 661 ccctggcctc ctactctttg ctcatcTTCT CCCTGGGGAG GCACACACGG CAGATGCTGC 721 AAAATGGGAC AAGCTCCAGA GATCCAACCA CTGAGGCCCA CAAGAGGGCC ATCAGAATCA 781 TCCTTTCCTT CTTCTTTCTC TTCTTACTTT ACTTTCTTGC TTTCTTAATT GCATCATTTG 841 GTAATTTCCT ACCAAAAACC AAGATGGCTA AGATGATTGG TGAAGTAATG ACAATGTTTT 901 ATCCTGCTGG CCACTCATTT ATTCTCATTC TGGGGAACAG TAAGCTGAAG CAGACATTTG 961 TAGTGATGCT CCGGTGTGAG TCTGGTCATC TGAAGCCTGG ATCCAAGGGA CCCATTTTCT 1021 CTGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1081 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1141 GGCTTTATAT ATCTTGTGGA AAGGACGAGC ACAGTCTCAG AGTGCCACAA CCACGCGTTA 1201 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt