Construct: ORF TRCN0000488929
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021333.1_s317c1
- DNA Barcode:
- GTCCTACGTCACATCGTTTAAACG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TP53I11 (9537)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488929
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9537 | TP53I11 | tumor protein p53 inducible... | NR_134612.2 | 10.4% | 1_2347del;2359G>A;2711_3475del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 435
- ORF length:
- 363
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcacaca ccagaagtgg cgagcccaag ccctggggac aggtgtaggg 121 agaaaagcag ccccaggccT CAGACTCGCT TCCCATCCTG GCATAGAGTG GGGGATGGCT 181 GGAGGGTGTC TATAGGTACA GCCCGCTCTG GCTGCTGCCA GGTGGGCCCC TGCCAGGGGT 241 CCTCACCCCT GTCCACCCTG TGCCTGGCTG TCCCTGCACC CAGATACAGC AACATGGCCT 301 GTACCCAGCA GAGTGGTGGC ACCACCATGG TTACAGCGGA TGCCCCGAGA CTCTGCTTGG 361 TAAACGTGGC AGAGCAGAAT GGGAGGCTGG ACCCTGAGGA AGGGCCCCTC TCCTGGCATC 421 TGTCTCTTGC TACCTAGAAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 481 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 541 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGTCCTAC GTCACATCGT 601 TTAAACGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt